SMARTer™ ICELL8® cx Single-Cell System User Manual
(061918)
takarabio.com
Takara Bio USA, Inc.
Page 2 of 35
Table of Contents
I.
Introduction ..................................................................................................................................................................... 5
A. Thank You for Your Order! ........................................................................................................................................ 5
B. About this Manual ....................................................................................................................................................... 5
C. Technical Support ....................................................................................................................................................... 5
D. ICELL8 cx Safety Information ................................................................................................................................... 6
II. System Description: Component Overview .................................................................................................................... 9
III.
System Description: Instrument Overview ............................................................................................................... 10
A. ICELL8 cx Specifications and Lab Requirements .................................................................................................... 13
B. Setup and Installation ................................................................................................................................................ 14
C. Required Equipment and Supplies from Your Lab or Other Suppliers .................................................................... 14
D. Required Materials from TBUSA ............................................................................................................................. 15
IV.
System Description: SmartChip Technology ............................................................................................................ 15
V. Protocol: Quick Reference Guide ................................................................................................................................. 16
VI.
Protocol: Preparing the ICELL8 cx System .............................................................................................................. 17
A. Powering on the System ............................................................................................................................................ 17
B. Checking System Containers .................................................................................................................................... 17
C. Running the Daily Warmup ...................................................................................................................................... 18
D. Running the Tip Clean Procedure ............................................................................................................................. 19
VII.
Protocol: Preparing the Source Plates ....................................................................................................................... 20
VIII. Protocol: Dispensing Samples into the Chip ............................................................................................................. 20
A. Selecting the SMARTer ICELL8 cx Application ..................................................................................................... 20
B. Placing the Chip in the ICELL8 cx Unit ................................................................................................................... 21
C. Placing the Sample Source Plate in the ICELL8 cx Unit .......................................................................................... 21
D. Dispensing the Sample Cells, Fiducial, and Controls into the Chip ......................................................................... 22
E. Imaging the Chip ....................................................................................................................................................... 25
F.
Blotting and Sealing the Chip ................................................................................................................................... 28
G. RT Dispense .............................................................................................................................................................. 29
H. Running your PCR on a Thermal Cycler .................................................................................................................. 32
I.
Cleaning the ICELL8 cx System............................................................................................................................... 32
IX.
Maintenance .............................................................................................................................................................. 33
A. Daily Maintenance .................................................................................................................................................... 33
B. Complete Shutdown Procedure ................................................................................................................................. 33
C. Common Replacement Parts ..................................................................................................................................... 33
