SMARTer™ ICELL8® cx Single-Cell System User Manual
(061918)
takarabio.com
Takara Bio USA, Inc.
Page 20 of 35
VII. Protocol: Preparing the Source Plates
A source plate is a 384-well plate containing either the samples (i.e., a sample source plate) or the PCR assays
(i.e., an assay source plate) that are to be transferred using the ICELL8 cx.
IMPORTANT:
Only the plates included in the SMARTer ICELL8 384-Well Source Plate
and Seal kit (Cat. No. 640192) are validated for use on the ICELL8 cx.
IMPORTANT:
Avoid introducing dust and debris to solutions that will be dispensed with
the ICELL8 cx. They can cause the tips to clog.
Observe the following precautions when assembling sample and assay source plates:
•
Consider assembling source plates in a dead air box to reduce environmental
dust
•
Wipe down the lab bench every day and wear gloves and a clean lab coat
•
Use plates, tips, and tubes from new or carefully covered containers
•
Work quickly and cover plates/tubes to minimize exposure to dust in the air
1.
Pipette the cell sample (for genotyping), Controls, and Fiducials into a 384-well sample source plate. Refer to
the plate map shown below (Figure 12).
Figure 12. Source plate map.
NOTE:
Use source plates immediately, or store on ice.
2.
After filling, seal the plate(s) with 384-Well Source Plate Seal(s). Do not centrifuge.
VIII. Protocol: Dispensing Samples into the Chip
A.
Selecting the SMARTer ICELL8 cx Application
1.
Click the
3’DE / TCR
tab (Figure 13).
Figure 13. Single Cell / TCR tab.
