Instrument Operating Manual
CyFlow® Cube 8 Rev-27_2015-07-02
Sysmex Partec GmbH
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Histogram and dot plot in immunology
Lysed blood 2D dot plot
Lysed blood is represented in a dot plot presenting the FSC in X axis and SSC in Y axis.
Both axes are in a linear scale.
Three distinct groups are visible; they represent the granulocytes (
G
), monocytes (
M
) and
lymphocytes (
L
).
Histogram and dotplot of immunological staining
This histogram represents the spectrum of the cells presented in the previous dot plot
stained with antibodies anti-CD45 conjugated to Phycoerythrin (PE) Dy647. The X axis
displays the fluorescence in a 4-dec logarithmic scale and the Y axis displays the number
of events in a linear scale.
This dot plot presents the cells fluorescence in X on a logarithmic
scale (CD45 PE-Dy647) versus SSC in Y on a linear scale.
This data display allows an easier interpretation of 2 parametric
data compared to the histogram. Concluded from this example,
the lymphocytes are strongly stained with the anti-CD45 antibody,
the monocytes are slightly stained and the population of
granulocytes is negative.
