Instrument Operating Manual
CyFlow® Cube 8 Rev-27_2015-07-02
Sysmex Partec GmbH
Am Flugplatz 13, 02828 Görlitz, Germany · Phone +49 3581 8746-0 · Fax +49 3581 8746-70 · [email protected] ·
www.sysmex-partec.com
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Analyze all (default selection)
This default mode will allow you to run your sample until its levels reached the stop
electrode.
Volumetric Counting with Volume
In the measure mode
volumetric counting with volume
the counting volume is flexible
and can be pre-selected by the user. In a first analysis phase the sample is acquired
normally as in continuous acquisition mode. Reaching the pre-selected volume the data
are cleared and the volumetric counting phase starts and the pre-selected volume will be
analyzed. The volume can be used as the basis for concentration determination.
Volumetric Counting with Electrodes
This measuring mode uses the START and STOP electrodes of the sample port to define
a fixed sample volume. In a standard sample por
t this “counting volume” is 200µl. In a
pre-counting phase the sample is acquired normally as in
continuous acquisition mode
.
Reaching the START electrode the data are cleared and the volumetric counting phase
starts. Reaching the STOP electrode the counting procedure will be terminated and a
system cleaning cycle will be initiated automatically.
Cells in Region
The
Events in Region
measure mode allows to define a number of particles within a
specified region to operate as STOP condition (select the respective
MaxCount
function
in the
REGIONS
register).
Working principle of the Absolute Volumetric Counting with Electrodes