Instrument Operating Manual
CyFlow® Cube 8 Rev-27_2015-07-02
Sysmex Partec GmbH
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particles resulting from sample preparation. In case of high signal amplification,
background can also be caused by particle contaminated sheath fluid.
The lower level (L-L) or threshold?
The lower level (L-L) threshold is a mean to suppress background signals. Signals below
the lower level are rejected from the signal acquisition. To exclude noise from a histogram
already acquired, a region-gate can be used.
Example of a histogram
A histogram represents a distribution of measured signals
(events) over 1 dimension. Data can be presented on the
dimensions of relative particle size or optical particle
structure (Forward Scatter (FCS) or Side Scatter (SSC)),
resp. or on their relative fluorescence intensities in
different light colors (fluorescence parameters FL1 to FL6).
In this example, the dimension represented is the relative
size (FSC) on a logarithmic scale in X, and the number of
events on a linear scale in Y. Two peaks are visible.
Example of a dot plot
A 2D dot plot presents correlated data over 2 dimensions. In the image on the right a
sample of leukocytes (after lyse of the red blood cells) is plotted with their relative light
scattering (SSC) property against the intensity of the CD45 antigen.
The Z value represents the number of events that have the same coordinates. 1 event will
be represented by a black point; if 10 events have the same coordinates, the point
representing them will be grey. It will be yellow if more than 20 points are overlaying one
another. The Z scale is dynamic and will adapt during the measurement to a scale of 1
(black) to the maximum overlaying event coordinate color-coded in red.