Siemens MicroSAM, Operating Instructions Manual, Page: 116 / 138

Glossary
MicroSAM
114
Operating Instructions, 06/2012, C79000-G5376-C560-07
&XW
Once the components have passed
through the first column, a number of them
are flushed through the cut output. They
never reach the second column. This
operating state is used in the following
cases:

To keep components which cannot be
backflushed away from the second
column, because these are followed by
further components which are to be
measured.

If components would falsify the result or
damage the second column.
 In order to directly pass components to
a further detector, e.g. to shorten the
duration of the analysis.
A.1.1 Advantages of live switching
● The sample only comes into contact with parts of the live T-piece, but not with valves.
● No switching valves are required in the oven, and the column switching does not result in
any temperature limitations.
● The live switching is maintenance free since there are no moving parts.
● The live switching has a minimum dead volume, and is therefore particularly suitable for
capillary columns.