Sartorius DCU 3, Operator'S Handbook Manual

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Operators Handbook DCU3 – System
5., Main Function „Calibration“
BAEDCU3 - Rev. 4.6 - 0504 5 - 5
5.4 Redox - Calibration
The Redox calibration is in fact a functional check. You will measure a calibration buffer and compare
the measured value with the reference indicated on the buffer bottle. If these values are different, the
Redox can be entered manually. The DCU - system then calculates a corresponding correction factor.
5.4.1 Operating Display
Tag Entry Displayed value or function, required input
REDOX mV Display of combined electrode voltage
CHECK mV Display of combined electrode voltage of last calibration
Start Check Electrode Enabling calibration of the Redox electrode
Additional touch keys, values and functions available in this display:
REFBU mV Enter reference voltage of redox buffer
5.4.2 Functional Check
The electrode function is checked before it is mounted into the culture vessel and therefore prior to the
sterilization. Since the heat impact of sterilization or chemical reactions with compounds of the culture
medium may effect the measuring characteristics of the electrode its function should be checked regu-
larly, i.e. prior to any next use and, if wrong operation is suspected, after a fermentation run.
1. Place Redox electrode in a beaker filled with the reference buffer
2. Enable functional check via „Start check electrode“. Follow the instructions of the menu.
5.4.3 Special Notes
If the measured buffer Redox and the value on the buffer bottle deviate by more than 6 mV
(about 3%) the electrode must be serviced. See documentation delivered with the electrode.