Riele 4040+, Operating Instructions Manual

Page: 33 / 73

4040+e_22.docx / 06.10.17
ROBERT RIELE GmbH & Co KG Page 33 6 - CALCULATION PROCEDURES
6.4.8 Calculation procedure 8 (C/S/SbRb)
Method at which the difference of reagent blank A
RB
and blank of reagent blank A
RBB
regarding the amount is
subtracted of the difference of sample value A
S
and sample blank A
SB
regarding the amount and this difference
is multiplied with a factor F which is determined by measuring of a standard solution with known concentration
C
ST
and under consideration of standard blank A
STB
and the difference of reagent blank A
RB
and blank of reagent
blank A
RBB
The sample blank A
SB
is measured before every test. The reagent blank A
RB
is entered or measured once.
Calculation procedure ....................................................... CP 8
Characteristic ........................................................ C / S / SbRb
Method ............................................... End Point with Standard
Calculation formula .............. C = F

( |A
S
- A
SB
| - |A
RB
- A
RBB
| )
Resulting factor ................... F = C
ST
/ ( |A
ST
-A
STB
| - |A
RB
-A
RBB
| )
Reagent blank ........................................ entering or measuring
PHOTOMETER
4040+ # 1001
V2.2a dd/mm/yy D
LAB.: RIELE BERLIN
USER 1: M.MUSTERMANN
DATE: 07/17/17
TIME: 08:44:12
METHOD 28: Ca
PROGRAM: 8
STANDARD: 8.02
WAVELENGTH: 546nm
TEMPERATURE: 37C
DELAY: 3s
MAX.UNITS: 12
UNIT: mg/dl
- - - - - - - - - - - -
MEASURE ZERO
Rb[A]: 0.150
Rbb[A]: 0.046
DELTA Rb: 0.104
ST[A] 1: 1.485
ST[A] 2: 1.521
ST[A] 3: 1.495
ST[A]: 1.501
STb[A]: 0.479
DELTA ST: 1.022
FACTOR: 8.74
NO. ABS. RESULT
1 1.495 7.89
Sb[A]: 0.489
2 1.542 7.89
Sb[A]: 0.535
3 1.394 8.39
Sb[A]: 0.329
Start method selection in the main menu.
See chapter:
4.1 - Measurement with programmed methods
4.2 - Measurement with basic methods
In the case of activated printer the print-out of the method data
follows.
The measuring window is shown.
Method procedure:

Insert / measure zero solution

Insert / measure blank of reagent blank

Insert / measure reagent blank
(Resulting blank)

Insert / measure standard blank

Insert / measure standard 1

Insert / measure standard 2 (optional)

Insert / measure standard 3 (optional)
(Averaged standard)
(standard blank)
(Averaged standard minus standard blank)
(Resulting factor)

Insert / measure sample blank

Insert / measure sample

Insert / measure sample blank

Insert / measure sample

Insert / measure sample blank

Insert / measure sample