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4040+e_22.docx / 06.10.17

ROBERT RIELE GmbH & Co KG

Page 40

6 - CALCULATION PROCEDURES

Calculation procedure ..................................................... CP 14
Characteristic ........................................................ C / F / Delta
Method ................................................... Difference with Factor
Calculation formula ..................... C = F



(



A

S2-Sb2

-



A

S1-Sb1

)

Factor ............................................................... given / entering
Sample blank ....................................................... with / without
Time / Delta  T1 .................................. entering (0, 10s to 255s)
Measure time  T2 ..................................... entering (0 to 1800s)
Delay  T3 .................................................. entering (0 to 1800s)
Reagent time #2 ....................................... entering (0 to 1800s)
Reagent time #3 ....................................... entering (0 to 1800s)

PHOTOMETER 4040+   # 1001
V2.2a  dd/mm/yy D
LAB.:   RIELE BERLIN
USER 1: M.MUSTERMANN
DATE:           07/17/17
TIME:           08:44:12
METHOD  14:    C/F DELTA
PROGRAM:              14
FACTOR:            1.000
WAVELENGTH:        405nm
TEMPERATURE:         37C
TIME/DELTA:          12s
MEASURE TIME:       100s
DELAY:               10s
REAGENT 2:           40s
REAGENT 3:           60s
UNIT:                U/l
- - - - - - - - - - - -
  MEASURE ZERO

NO.    Sb[A]     S[A]E1
1    0.083    0.411
2    0.110    0.382
3    0.146    0.492

NO.    Sb[A]     S[A]E2
1    0.091    1.090
2    0.140    0.991
3    0.200    1.165

NO.   RESULT
1    0.671
2    0.578
3    0.619

NO.    Sb[A]     S[A]E1
1    0.000    1.012
2    0.000    1.138
3    0.000    1.076

NO.    Sb[A]     S[A]E2
1    0.000    1.458
2    0.000    1.530
3    0.000    1.384

NO.   RESULT
1    0.446
2    0.392
3    0.307

Start method selection in the main menu.
See chapter:
4.1 - Measurement with programmed methods
4.2 - Measurement with basic methods

In the case of activated printer the print out of the method data
follows.

The measuring window is shown.

Method procedure with sample blank:



Insert / measure zero solution



Measure all samples E1 (maximum 25)



Change to measuring E2 by [E1/E2]



Measure all samples E2 (maximum 25)

Results based on the differences of the measured samples



Show the results by [MODE] [MODE] [DETAIL]

Method procedure without sample blank:



Measure all samples E1 (maximum 25)



Change to measuring E2 by [E1/E2]



Measure all samples E2 (maximum 25)

Results based on the differences of the measured samples



Show the results by [MODE] [MODE] [DETAIL]

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Summary of Contents for 4040+

Page 1: ...Operating Instructions Photometer 4040 ROBERT RIELE GmbH Co KG Software Version 2 2 Documentation Version 08 2017 ...

Page 2: ... for the marking of important information for appropriate handling of the device Biohazard Samples containing material of human origin must be treated as potentially infectious The relevant laboratory guidelines on safe use must be observed Symbol for the marking of electrical and electronics devices according to 7 ElektroG IP XO No special protection against penetrating moisture IP International ...

Page 3: ...ium battery may be carried out only by authorized specialist staff Through improper repairs the warranty extinguishes and the operator can be heavily jeopardized If suspected the device can no longer be operated safely turn it off and take steps to ensure that no one will subsequently attempt to use it Electromagnetic waves Devices that emit electromagnetic waves may affect measured data or cause ...

Page 4: ... the accessories can be given back to the manufacturer with costs for an environmental waste disposal The previous professional decontamination has to be proved with a certificate Address of the manufacturer ROBERT RIELE GmbH Co KG Kurfuerstenstrasse 75 79 D 13467 BERLIN GERMANY Phone 49 0 30 404 40 87 Fax 49 0 30 404 05 29 E mail info riele de www riele de QUALITY MANAGEMENT SYSTEM ROBERT RIELE G...

Page 5: ...to the methods with multi standards 18 6 1 6 Fundamental to bichromatic measurements 18 6 1 7 Fundamental to the Kinetic 19 6 1 8 Fundamental to the methods with reagent blank 22 6 1 9 Fundamental to ID NO and sample numerator 22 6 1 10 Fundamental to storing test results 22 6 2 ABBREVIATIONS 23 6 3 SURVEY OF THE METHODS 24 6 4 DESCRIPTION OF METHOD PROCEDURES 25 6 4 1 Calculation procedure 1 C F ...

Page 6: ...4 Touchscreen adjustment 59 8 2 15 Date Time 60 8 2 16 Language 60 8 2 17 ADC counts Optic 61 8 2 18 Service tools 61 9 MAINTENANCE 62 9 1 CLEANING INSTRUCTION 62 9 2 CALIBRATING MEASURING SYSTEM 62 9 3 REPLACEMENT OF PAPER ROLL 62 9 4 REPLACEMENT OF LINE FUSES 63 10 ERROR MESSAGE CORRECTION 64 10 1 GENERAL NOTE 64 10 2 ACOUSTIC ERROR MESSAGES 64 10 3 PLAINTEXT ERROR MESSAGES 64 10 4 CODED ERROR M...

Page 7: ...otometer 4040 For measuring methods several programmed methods with open parameters are available chapter 6 CALCULATION PROCEDURES and chapter 13 METHOD LIST Besides up to 231 methods built up on the basic methods can be established and stored by the operator with a method editor A list of methods can be printed out chapter 7 METHOD EDITOR In modus 1 up to 50 nonlinear calibration curves with maxi...

Page 8: ... the device If the device was exposed to extraordinary fluctuation in temperature and or humidity it must acclimatize suffi ciently before operation 2 3 INSTALLATION Photometer 4040 operates at any line voltage between 100 VAC and 240 VAC at 50 60 Hz The device plug of the mains cable must be put into the socket at the back of the device and the mains plug into a grounded mains socket While connec...

Page 9: ...er 10 ERROR MESSAGE CORRECTION 2 4 LOADING PRINTER PAPER With initial operation or if the colored end of the paper roll appears printer paper must be inserted Open the printer cover Put the green head up lever in the up position Remove the rest of paper Put the green head up lever in the down position Put printer paper axis into the new printer paper reel Insert the paper inside the printer The ro...

Page 10: ...4040 e_22 docx 06 10 17 ROBERT RIELE GmbH Co KG Page 10 3 OPERATING ELEMENTS 3 OPERATING ELEMENTS 3 1 FRONT 3 2 BACK working area printer cover touchscreen lid ...

Page 11: ...another pointed article 3 4 WORKING AREA 3 5 CUVETTE COMPARTMENT 3 5 1 Working with standard cuvettes The optical path is directed from the back to the front of the device Insert single cuvette according to the drawing OPTIC CONSTRUCTION in TECHNICAL DATA Trigger setting to zero by ZERO Trigger a normal measuring by RESULT 3 5 2 Reducing the minimum measuring volume The minimum measuring volume ca...

Page 12: ...ical compartment is displayed as o37 0 C and of an optional attached incubator i37 0 C In the case of switched off tempering the temperatures are not displayed In the case of switched on tempering and instable temperature the display changes between C and e g 37 3 C In the case of stable temperature the current temperature of e g 37 0 C is shown Small fluctuations of the value are normal Date in t...

Page 13: ...he following abbreviations are used for the distinction of the methods CONC concentration KIN kinetic FTK fixed time kinetic F factor STD standard RB reagent blank SB sample blank Further information Chapter 6 CALCULATION PROCEDURES 4 3 Method editor Each photometric test can be permanently stored with its setting parameters by the method editor With the functions of the method editor are possible...

Page 14: ...s 4040 Further information Chapter 8 UTILITY PROGRAMS 4 5 Line feed LF Pressing LF in the main menu triggers a line feed in the case of activated printer Several lines can be advanced by continuous pressure on LF U T I L I T I E S P A G E 1 5 O P T I C A D J U S T M E N T P A G E M U L T I S T A N D A R D P R I N T E R B A R C O D E E X I T o 3 7 0 C i 3 7 0 C 0 7 2 4 2 0 1 7 1 0 1 4 ...

Page 15: ... are displayed The current position of the reading unit is underlaid with a white background Here at position 3 the result is currently measured Push ESC to exit the four channel modus By pushing BC either a barcode may be read when a barcode reader is connected the barcode is underlaid with a white background or a barcode is generated in the format YYMMDD0000001 including the year month day YYMMD...

Page 16: ...s are interrupted and the following window is reached ESC terminates the four channel modus 2 M returns to the measurements Interrupted measurements are continued However original reaction times are not valid anymore With P1 P2 and P3 more details of the tests in channels 1 3 may be shown The progress of the kinetic is shown after a few seconds The axis of time is marked by s the axis of extinctio...

Page 17: ... shown at the Iower edge of the touchscreen For meaning of the display see chapter 4 PROGRAM SELECTION MAIN MENU A temperature instable or out of tolerance during measuring is marked by an asterisk at the utmost right position in the corresponding print line To avoid deviations due to temperature influence a delay between triggering and actual measuring can be programmed in each method For a quick...

Page 18: ... can also be expanded to all measurements The correspond ing entry can be set invoking a basic method The parameter is definable in preprogrammed methods chapter 7 METHOD EDITOR 6 1 5 Fundamental to the methods with multi standards Linear calibration is used in the case of two calibrators The absorbance forms a linear diagram with the concentrations chapter 8 2 2 Multi standard functions Nonlinear...

Page 19: ...prove the linearity of the kinetic only the best three deltas are considered in the calculation procedure of the regression calculation Therefore at least three deltas are required when programming a new method If this procedure does not lead to an improvement all deltas are reconsidered in the calculation procedure In practice linear tests show values of R 2 near to 1 In the example for Calculati...

Page 20: ...measurement is repeated The measurement is repeated by RETRY View after pressing DIAG The progress of the kinetic is shown after a few seconds The axis of time is marked by s the axis of extinction is marked by ABS The currently used FACTOR and the calculated DELTA REG are shown in the bottom lines If R 2 is activated the term LINEAR or NON LINEAR is shown in the upper right corner By PRINT a grap...

Page 21: ...0 4 90 0 767 5 120 0 785 6 150 0 805 7 180 0 825 ABS 1 0 642 ABS 2 0 750 1 0 0312 2 0 0320 3 x 0 0345 4 x 0 0364 5 x 0 0387 6 0 0417 DELTA REG 0 0365 ABS ABS 2 ABS 1 RESULT DELTA REG x FACTOR Printout after confirming DIAGR and PRINT The chronic of the kinetic is printed The axis of time is marked by s the axis of extinction is marked by ABS In the next step a chart including all data points is pr...

Page 22: ...ts are labeled with a numerator Additionally all results can be labeled with a 5 digit ID NO When the ID NO is not zero it will be displayed and printed together with the sample result When a method is selected you can edit both numerator and ID NO of a sample with MODE NUM Press N I to switch between editing numerator and ID NO 6 1 10 Fundamental to storing test results All test results are store...

Page 23: ...reaction time T1 ASTB Absorbance of standard blank C Concentration CST Concentration of standard CV Quality control Coefficient of variation dA min At Kinetic ΔA min ARB Minit At Kinetic change of reagent blank per minute measured in A min AS Minit At Kinetic change of sample per minute measured in A min F Factor FTK Fixed Time Kinetic KIN Kinetic n Quality control number of values nm Nanometer di...

Page 24: ...ndpoint with Standard C F AS CP 6 C S Rb Endpoint with Standard C F AS ARB CP 7 C S Sb Endpoint with Standard C F AS ASB CP 8 C S SbRb Endpoint with Standard C F AS ASB ARB ARBB CP 9 FTK F Rb Fixed Time Kinetic with Factor C F AS 0 AS 1 ARB 0 ARB 1 CP 10 FTK S Rb Fixed Time Kinetic with Standard C F AS 0 AS 1 ARB 0 ARB 1 CP 11 KIN F Rb Kinetic with Factor C F AS Minit ARB Minit CP 12 KIN S Rb Kine...

Page 25: ...rminated MODE Occupies the action keys with following mode functions NUM MODE LF QC RETURN PRN DETAIL M STD RETURN PRN DETAIL ZERO RETRY RETURN PRN DETAIL ZERO E1 E2 RETURN ZERO Starts the zero measuring RESULT Starts the measuring Mode functions NUM Edit sample numerator or ID NO chapter 6 1 9 LF Line Feed QC Quality Control functions PRN Switch printer ON OFF printout of current time by TIME DET...

Page 26: ... mm yy D LAB RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 20 HEMOGLOBIN PROGRAM 1 FACTOR 29 4 WAVELENGTH 405nm TEMPERATURE 37C DELAY 5s MAX UNITS 25 UNIT g l MEASURE ZERO NO ABS RESULT 1 0 675 19 8 2 0 843 24 8 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the pr...

Page 27: ...ng PHOTOMETER 4040 1001 V2 2a dd mm yy D LAB RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 21 HDL C PROGRAM 2 FACTOR 325 WAVELENGTH 546nm TEMPERATURE 37C DELAY 5s UNIT mg dl MEASURE ZERO Rb A 0 058 NO ABS RESULT 1 1 064 327 2 1 188 367 3 1 340 417 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In ...

Page 28: ... USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 23 BILIRUBIN PROGRAM 3 FACTOR 12 80 WAVELENGTH 546nm TEMPERATURE 37C DELAY 5s MAX UNITS 8 0 UNIT mg dl MEASURE ZERO NO ABS RESULT 1 1 000 4 21 Sb A 0 671 2 1 215 4 25 Sb A 0 884 3 1 033 4 23 Sb A 0 702 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of ...

Page 29: ...agent blank entering or measuring PHOTOMETER 4040 1001 V2 2a dd mm yy D LAB RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 24 Fe PROGRAM 4 FACTOR 1330 WAVELENGTH 578nm TEMPERATURE 37C DELAY 5s MIN UNITS 37 MAX UNITS 158 UNIT ug dl MEASURE ZERO Rb A 0 085 Rbb A 0 198 DELTA Rb 0 113 NO ABS RESULT 1 0 715 154 Sb A 0 486 2 0 646 49 Sb A 0 497 Start method selection in the main men...

Page 30: ...METHOD 25 GLUCOSE PROGRAM 5 STANDARD 5 55 WAVELENGTH 546nm TEMPERATURE 37C DELAY 3s MAX UNITS 22 2 UNIT mmol l MEASURE ZERO ST A 1 1 110 ST A 2 1 093 ST A 3 1 059 ST A 1 088 FACTOR 5 10 NO ABS RESULT 1 1 026 5 23 2 1 357 6 92 3 1 582 8 07 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer t...

Page 31: ...AB RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 26 SODIUM PROGRAM 6 STANDARD 150 0 WAVELENGTH 405nm TEMPERATURE 37C DELAY 3s MAX UNITS 300 UNIT mmol l MEASURE ZERO Rb A 0 108 ST A 1 1 112 ST A 2 1 132 ST A 3 1 118 ST A 1 121 FACTOR 148 2 NO ABS RESULT 1 1 449 198 7 2 1 118 149 6 5 2 006 281 2 Start method selection in the main menu See chapter 4 1 Measurement with programmed...

Page 32: ...D 50 0 WAVELENGTH 546nm TEMPERATURE 37C DELAY 3s MAX UNITS 220 UNIT mg dl MEASURE ZERO ST A 1 0 614 ST A 2 0 629 ST A 3 0 620 ST A 0 621 STb A 0 106 DELTA ST 0 515 FACTOR 97 1 NO ABS RESULT 1 2 292 197 6 Sb A 0 257 2 2 340 198 0 Sb A 0 300 3 2 223 197 2 Sb A 0 193 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the c...

Page 33: ... RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 28 Ca PROGRAM 8 STANDARD 8 02 WAVELENGTH 546nm TEMPERATURE 37C DELAY 3s MAX UNITS 12 UNIT mg dl MEASURE ZERO Rb A 0 150 Rbb A 0 046 DELTA Rb 0 104 ST A 1 1 485 ST A 2 1 521 ST A 3 1 495 ST A 1 501 STb A 0 479 DELTA ST 1 022 FACTOR 8 74 NO ABS RESULT 1 1 495 7 89 Sb A 0 489 2 1 542 7 89 Sb A 0 535 3 1 394 8 39 Sb A 0 329 Start met...

Page 34: ...ain Calculation procedure CP 9 Characteristic FTK F Rb Method Fixed Time with Factor Calculation formula C F AS 0 AS 1 ARB 0 ARB 1 Factor given entering Reagent blank entering or measuring PHOTOMETER 4040 1001 V2 2a dd mm yy D LAB RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 29 CK MB PROGRAM 9 FACTOR 2751 3 WAVELENGTH 340nm TEMPERATURE 37C INCUBATION 120s REACTION 180s MAX U...

Page 35: ...0 Characteristic FTK S Rb Method Fixed Time with Standard Calculation formula C F AS 0 AS 1 ARB 0 ARB 1 Resulting factor F CST AST 0 AST 1 ARB 0 ARB 1 Reagent blank entering or measuring PHOTOMETER 4040 1001 V2 2a dd mm yy D LAB RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 30 CREATININ PROGRAM 10 STANDARD 2 00 WAVELENGTH 492nm TEMPERATURE 37C INCUBATION 45s REACTION 60s MAX ...

Page 36: ...dd mm yy D LAB RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 31 GOT PROGRAM 11 FACTOR 1746 0 WAVELENGTH 340nm TEMPERATURE 37C DELAY 60s DELTAS 5 TIME DELTA 18s MAX UNITS 280 MIN R 2 0 998 UNIT U l MEASURE ZERO Rb A 0 000 DELTA Rb 0 000 R 2 0 9762 NO ABS RESULT 1 0 123 189 R 2 0 9996 2 0 154 189 R 2 0 9993 3 0 209 96 R 2 1 0000 Start method selection in the main menu See chapt...

Page 37: ...ERMANN DATE 07 17 17 TIME 08 44 12 METHOD 32 UREA PROGRAM 12 STANDARD 80 0 WAVELENGTH 340nm TEMPERATURE 37C DELAY 3s DELTAS 5 TIME DELTA 5s MIN R 2 0 998 UNIT mg dl MEASURE ZERO Rb A 0 000 Rb KIN 0 000 R 2 0 1973 ST KIN 1 0 327 R 2 0 9996 ST KIN 2 0 330 R 2 0 9989 ST KIN 3 0 324 R 2 0 9994 ST KIN 0 327 FACTOR 244 3 NO ABS RESULT 1 0 232 41 5 R 2 0 9984 2 0 175 81 8 R 2 0 9997 Start method selectio...

Page 38: ... 13 TRANSM PROGRAM 13 FACTOR 1 0 WAVELENGTH 546nm TEMPERATURE 37C DELAY 2s UNIT MEASURE 100 NO ABS RESULT 1 0 329 46 9 2 1 004 9 9 3 2 020 1 0 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the print out of the method data follows The measuring window is shown Method procedure Insert me...

Page 39: ...ample blank is queried After the blank is measured the time controlled measuring process will be started with RESULT With a combination of acoustic signals and text messages on the display the Photometer takes control of the timing for the whole measuring The measurement of the samples E1 can be finished at any time with E1 E2 In the second course will be so many samples measured as in the first c...

Page 40: ... Sb A S A E2 1 0 091 1 090 2 0 140 0 991 3 0 200 1 165 NO RESULT 1 0 671 2 0 578 3 0 619 NO Sb A S A E1 1 0 000 1 012 2 0 000 1 138 3 0 000 1 076 NO Sb A S A E2 1 0 000 1 458 2 0 000 1 530 3 0 000 1 384 NO RESULT 1 0 446 2 0 392 3 0 307 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of activated printer the...

Page 41: ...ation formula C mg dl F 168 A415nm 84 A380nm 84 A450nm Factor given entering Conversion factor µmol L 0 6206 mg dl PHOTOMETER 4040 1001 V2 2a dd mm yy D LAB RIELE BERLIN USER 1 M MUSTERMANN DATE 07 17 17 TIME 08 44 12 METHOD 15 C F 3 WL PROGRAM 15 FACTOR 1 00 WAVELENGTH 380 415 450nm TEMPERATURE 37C DELAY 2s UNIT mg dl MEASURE ZERO NO µmol L mg dl 1 4 859 7 83 2 5 865 9 45 Start method selection i...

Page 42: ... the first pipetting phase During the procedure the user is guided by indications on the screen draw up and dispense reagent etc see Fig 5 4 16 2 and acoustic signals The factor Fdil is the volume correction factor which is calculated on the basis of the given volumes sample volume a R1 volume b and R2 volume c on page 3 3 of the method parameters The factor is set to 1 000 by default when given v...

Page 43: ...DELTA R1R2 PROGRAM 16 FACTOR 1 000 WAVELENGTH 546nm TEMPERATURE 37C TIME DELTA 30s DELAY 0s TIME REA 1 130s TIME REA 2 130s UNIT MEASURE ZERO E1 1 0 285 A E1 2 0 285 A E1 3 0 285 A E2 1 0 165 A E2 2 0 165 A E2 3 0 165 A NO RESULT 1 0 116 2 0 116 3 0 116 Start method selection in the main menu See chapter 4 1 Measurement with programmed methods 4 2 Measurement with basic methods In the case of acti...

Page 44: ... procedure see 6 3 SURVEY OF THE METHODS In Fig 6 4 all setting parameters can be edited METHODE DELETE Change to Fig 6 3 where the number of the method to be deleted is queried After a prompt for confirmation the selected method is deleted Basic and fixed methods cannot be deleted LIST A list of all programmed methods can be printed and transmitted via the serial interface EXIT Return to main men...

Page 45: ...target method P 3 Change to next parameter window Specifics in parameter window 3 At least one control serum must be defined before data can be entered see chapter 8 2 6 Quality control If at least one control serum with its setpoint and range is entered corresponding memory of the quality control is reserved for this method So it can be supervised with integrated quality control If both ID identi...

Page 46: ...with multi standard there is the editor window for the curve bases P and P Consecutive numbering of the current bases A C Switch input between A for absorbance and C for concentration E Accept the edited value Input and confirmation of a single 0 at A lead to the deletion of the current pair of points In order to set the value to zero enter e g 0 0 ESC End input and save curve data For measuring i...

Page 47: ...selected by pressing the relating key Utilities Description in chapter Optic adjustment 8 2 1 Multi standard functions 8 2 2 Printer ON OFF 8 2 3 Bar Code 8 2 4 Menu serial COM 8 2 5 Quality control 8 2 6 Settings printout 8 2 7 Stored results 8 2 8 Heating ON OFF 8 2 9 Laboratory name 8 2 10 User name 8 2 11 Error list 8 2 12 Key signal ON OFF 8 2 13 Touchscreen adjustment 8 2 14 Date Time 8 2 15...

Page 48: ...ange light the lid has to be closed during the optic adjustment Start the optic adjustment by START Calibration of the dark level Wait for about 40s until adjustment is finished The function cannot be interrupted After completion the program returns to the utility program level Monthly executed the optic adjust ment compensates possible deviations of the measuring accuracy due to environmental inf...

Page 49: ...asuring the multi standards branches out automatically to the calculation procedures as follows CP1 CP5 CP2 CP6 CP3 CP7 CP4 CP8 CP9 CP10 CP11 CP12 CP14 CP14 Print out of curve For measuring in another standard method than suggested start measuring multi standards in each other selected standard method before measuring the first standard by MODE MODE M STD Curve number and standards are queried acc...

Page 50: ...gle 0 at A lead to the deletion of the current pair of points In order to set the value to zero enter e g 0 0 ESC End input and save curve data The bases are sorted in ascending order according to their A value 8 2 3 Printer ON OFF The current status of the internal printer is indicated in the first line by OFF or ON Change setting by SELECT Save setting permanently by OK Save setting temporarily ...

Page 51: ...rate 8 2 4 1 EDP ON OFF The current status of the EDP Electronic Data Processing interface is indicated in the first line by OFF or ON Change setting by SELECT EDP OFF no output EDP ON CR LF system output through serial port with CR LF protocol EDP ON STX ETX BCC system output through serial port with STX ETX BCC protocol EDP ON CR LF LOG after each measurement a formatted string will be output th...

Page 52: ...6 control serums Each QC supervised method can be connected with 2 control serums The QC data of a series of measurements are stored in a daily memory Each reading is stored with method number date and user identification From the daily memory the individual QC data can become deleted or saved in the monthly memory of the corresponding method The monthly memory of a QC method can record up to 31 r...

Page 53: ... for serum 1 DAILY MEMORY S1 PHOTOMETER 4040 1001 V2 2a dd mm yy D LAB RIELE BERLIN DATE 07 17 17 Measuring data of the corresponding daily memory are shown with method number method name reading and dimension Change to next reading Change to previous reading DEL Delete shown reading in the daily memory and confirm again by DEL STORE Store shown reading in the monthly memory and confirm again by S...

Page 54: ...the reading lies within minus 1s From 3s the warning level starts With a deviation of 3s an is displayed For the calculation of the quality values of a method at least 20 readings in the month memory must be present 1 User identification The keys have following functions Change to next reading Change to previous reading DEL Delete all measuring data of the monthly memory of the selected method and...

Page 55: ...2 05 10 12 74 2s 1 02 04 10 12 13 2s 1 02 03 10 12 65 2s 2 02 02 10 13 11 1s 1 02 01 10 13 88 1s 3 01 31 10 13 51 1s 3 01 30 10 13 24 1s 3 01 29 10 12 50 2s 1 01 28 10 12 74 2s 2 01 27 10 13 30 1s 1 Output dialog If at least 20 readings are stored in the monthly memory these are indicated in the Levey Jennings plot In this representation the deviations can be controlled visually and thus tendencie...

Page 56: ...1 340 17 TEMPERATURE OPTIC 37C 14566 BATTERY OK ADC CORRECTION 250 EDP ON CR LF TOUCH Mx148 My192 Fx120 Fy100 LANGUAGE 1 ENGLISH 2 GERMAN KEY SIGNAL ON M COUNTER 351 T COUNTER 334 45 S COUNTER 402 ADJUST OPTIC POSITION P3 1 350 3850 7246 D2 3 3500 D1 2 3396 PROGR METHODS 0 STORED RESULTS 0 By START the program version and the complete status of the saved settings are printed out The percentage is ...

Page 57: ...device by EXIT 8 2 10 Laboratory name The name of the laboratory can be stored permanently In case of a stored name an additional line within the header is sent to the printer or to the EDP By NEW the entry of the laboratory name is possible Enter the laboratory name via the alphanu meric keyboard Following functions are available a 1 change to lowercase 1 A change to numeric characters A a change...

Page 58: ...t error is shown first The last error is always marked with no 1 By NEXT earlier error messages are shown By PRINT the complete error list is printed or output to the serial interface For troubleshooting the coded error list can be consulted chapter 10 4 CODED ERROR MESSAGES 8 2 13 Key signal ON OFF The current status of the key signal is indi cated in the first line by OFF or ON Change setting by...

Page 59: ...pper corner Touch the intersection point After a timeout of 10s the memory inquiry follows Save the adjustment by OK Reject the adjustment by EXIT Hint If the device is maladjusted this function can directly be called during the switching on routine Switch on the device After greeting screen chapter 2 3 INSTALLATION is displayed keep the touchscreen pressed After some seconds a deep signal tone so...

Page 60: ... ABS RESULT 1 0 675 19 8 TIME 11 21 32 When printer ON via MODE PRN after a measurement the current time can be printed out by TIME 8 2 16 Language The current status of the language is indicated in the first line The setting can be changed by SELECT Following options are possible LANGUAGE ENGLISH LANGUAGE GERMAN Save setting permanently by OK The setting is temporarily saved up to next switch off...

Page 61: ...the dark level adjustment The function L switches the LED off and on CH switches between the channels 1 3 and F The filter positions are disappearing when channels 1 3 are selected P1 3 shows the values of channel 1 3 P0 is the corresponding dark position of the 340 nm channels Stop function by ESC 8 2 18 Service tools The service tools are reserved for trained specialists only and therefore prote...

Page 62: ...evice other than those specified Consult the laboratory protocol for handling biohazardous materials Take care that no liquid enters the device There is no protection against penetrating of liquids Code IP X0 For device cleaning and surface decontamination purposes use commercial decontaminating solution which are usually available in clinical chemistry laboratories like Mikrozid AF Liquid Bacillo...

Page 63: ...ed on the rear panel To replace those fuses unplug the mains cable and remove the fuse holder with the fuses as shown below The instrument is delivered with two spare fuses Neither use makeshift fuses nor short circuit the fuse holder Specifications of mains fuse dimensions mm 5 20 standard IEC 60127 2 V time current characteristic time lag T voltage rating 250 V rated current 1 6 A marking T 1 6 ...

Page 64: ...ry 10 3 PLAINTEXT ERROR MESSAGES RANGE MIN The programmed low limit was under run by the measurement RANGE MAX The programmed upper limit was exceeded by the measurement NON LINEAR The square of the correlation coefficient r lies at the kinetic measuring below the programmed low limit RANGE At the kinetic measuring the procedure of the kinetic is wrong increasing falling NO METHOD Dialed method is...

Page 65: ...ck measuring solution 23 overflow at Kinetic check measuring solution 24 overflow at Kinetic check measuring solution 25 overflow at Kinetic check measuring solution 26 overflow at Kinetic check measuring solution 27 overflow at Kinetic check measuring solution 28 overflow at Kinetic check measuring solution 29 wrong input of deltas or time per delta restart method 30 battery empty contact service...

Page 66: ...rds 64 current method not found in monthly memory check QC data of method 65 more than 50 QC methods defined delete unused QC methods 66 internal clock is off QC data not storable switch on internal clock 67 BCC error in dataset of QC method values check current method 68 free monthly memory not found at QC delete unused QC methods 69 free space in daily memory not found at QC empty QC daily memor...

Page 67: ...from excessive dust Free from combustible gases Free from vibrations Free from electromagnetic wave interference Well distanced from a machine generating a high frequency high voltage e g a centrifuge 11 2 MINIMAL OPERATION QUALITY Signal processing in analogue amplifiers with high amplification factors cannot differentiate desired from undesired signals Amplifiers thus are apt to be overloaded or...

Page 68: ...n system with capacity for up to 231 programmable methods Data import by touchscreen or PC Up to 50 nonlinear calibration curves with max 20 sets of points can be stored Signal Port Serial port for connection to an external printer and or PC Data Logging Up to 1000 results can be saved in the memory automatically Measurement Procedures Absorbance End point with factor standard or multiple standard...

Page 69: ...g System B 1 1 Optical configuration cf Figure B 1 2 Source s of radiation LED B 1 3 Spectroscopic apparatus filter wheel B 1 4 Radiation detector s photodiode B 1 5 Cuvette s cell s 10 mm glass or plastic cuvette square shaped B 1 6 Temperature regulation of the cuvette 37 C B 1 7 Displayed units absorbance mass concentration enzyme activity B 1 8 Display device digital display absorbance 0 000 t...

Page 70: ...ound pressure level SPL 50 dB B 5 Maximum permissible errors and other limiting values B 5 1 Photometric uncertainty of the spectral absorbance max 3 B 5 2 Photometric short time variation coefficient 1 B 5 3 Uncertainty of wavelengths max 2 nm B 5 4 Spectral half width of spectral radiation flux at detector 10 nm B 5 5 Percentage of wavelength integrated false radiation measured at 340 nm as tran...

Page 71: ... cuvettes 1000 pcs 1704818001 Dust cover 5010 018 Fuses for line power 10 pcs 5010 024 Biogent A 1000 ml 500 002 Incubator T12 500 001 Incubator T16 500 005 Incubator T20 0552402001 Mains cable 5010 005 Operator s manual 090073 Printer paper 5 rolls 090 064 Secondary calibration standards four piece certified 4040 034 Spacer for 250 µl measuring volume 4040 035 Spacer for 500 µl measuring volume 1...

Page 72: ... 13 METHOD LIST 1 16 16 basic methods chapter 13 1 BASIC METHOD 17 19 free reserved for further 3 automatic calculation methods 20 250 up to 231 user specific methods chapter 13 2 LIST OF USER SPECIFIC METHODS as copy master to be filled out by the user 13 1 BASIC METHODS ...

Page 73: ...4040 e_22 docx 06 10 17 ROBERT RIELE GmbH Co KG Page 73 13 METHOD LIST 13 2 LIST OF USER SPECIFIC METHODS ...

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