4040+e_22.docx / 06.10.17
ROBERT RIELE GmbH & Co KG
Page 36
6.4.11 Calculation procedure 11 (KIN/F/Rb)
Method at which a sample S is measured several times (depending on the number of deltas) in an equidistant
time grid. From the resulting absorbance values an alteration per minute
A
S,Minute
is determined by a linear
regression calculation. The reagent blank
A
RB,Minute
is measured in the same way as the sample (or entered
directly in U/l) and subtracted from the sample value. This difference is multiplied by a given factor F. When
running a kinetic test with decreasing absorbance the factor F should be minus signed (e.g. F = -1746) in order
to get a positive result. The factor F should be positive for tests with increasing absorbance.
During the procedure the dialog asks for the use of a reagent blank. The default value is OFF. To continue with-
out reagent blank press [ENTER].
Calculation procedure ..................................................... CP 11
Characteristic ......................................................... KIN / F / Rb
Method ........................................................ Kinetic with Factor
Calculation formula ..................... C = F
(
A
S,Minit
-
A
RB,Minit
)
Factor ............................................................... given / entering
Reagent blank ........................................ entering or measuring
Number of deltas ........................................... entering (3 to 19)
Time per delta ........................................ entering (4 s to 255 s)
PHOTOMETER 4040+ # 1001
V2.2a dd/mm/yy D
LAB.: RIELE BERLIN
USER 1: M.MUSTERMANN
DATE: 07/17/17
TIME: 08:44:12
METHOD 31: GOT
PROGRAM: 11
FACTOR: -1746.0
WAVELENGTH: 340nm
TEMPERATURE: 37C
DELAY: 60s
DELTAS: 5
TIME/DELTA: 18s
MAX.UNITS: 280
MIN.R^2: 0.998
UNIT: U/l
- - - - - - - - - - - -
MEASURE ZERO
Rb[A]: 0.000
DELTA Rb: 0.000
R^2: 0.9762
NO. ABS. RESULT
1 0.123 189
R^2: 0.9996
2 0.154 189
R^2: 0.9993
3 0.209 96
R^2: 1.0000
Start method selection in the main menu.
See chapter:
4.1 - Measurement with programmed methods
4.2 - Measurement with basic methods
In the case of activated printer the print-out of the method data
follows.
The measuring window is shown.
This example shows a negative factor producing a positive result
at decreasing absorbance.
The absorbance value will be constantly refreshed on the display
during the delay time.
At the beginning and at the end of the delay time, the absorbance
values ABS.1 and ABS.2 are measured respectively.
Method procedure:
Insert / measure zero solution
Without reagent blank (insert / measure optionally)
Insert / measure sample
Numerator / |ABS.1
– ABS.2| / Result
R^2: coefficient of determination, used for linearity control of the
test (see chapter 6.1.7 - Fundamental to the K
Insert / measure sample
Insert / measure sample
Detail print-out of ABS.1, ABS.2 and deltas.
A detail print-out of ABS.1, ABS.2 and deltas can be made after
each measurement with [MODE] [MODE] [DETAIL] (see chapter
6.1.7 Fundamental to the K
