4040+e_22.docx / 06.10.17
ROBERT RIELE GmbH & Co KG
Page 41
6.4.15 Calculation Procedure 15 (C/F 3 WL)
Method at which a sample is measured with three different wavelengths: 380 nm, 415 nm and 450 nm. This
method is appropriated for free hemoglobin measurements.
The mentioned wavelengths are not included in the standard set of filters.
The factor has to be adjusted if using thinner (see chapter 6.1.3).
Calculation procedure ..................................................................................... CP 15
Characteristic ............................................................................................ C / F 3 WL
Method .................................................................. Measurement with 3 wavelengths
Calculation formula .............. C [mg/dl]= F * (168 * A
415nm
– 84 * A
380nm
– 84 * A
450nm
)
Factor ................................................................................................. given / entering
Conversion factor ............................................................... µmol/L
≡ 0,6206 * mg / dl
PHOTOMETER 4040+ # 1001
V2.2a dd/mm/yy D
LAB.: RIELE BERLIN
USER 1: M.MUSTERMANN
DATE: 07/17/17
TIME: 08:44:12
METHOD 15: C/F 3 WL
PROGRAM: 15
FACTOR: 1.00
WAVELENGTH:
380/415/450nm
TEMPERATURE: 37C
DELAY: 2s
UNIT: mg/dl
- - - - - - - - - - - -
MEASURE ZERO
NO. µmol/L mg/dl
1 4.859 7.83
2 5.865 9.45
Start method selection in the main menu.
See chapter:
4.1 - Measurement with programmed methods
4.2 - Measurement with basic methods
In the case of activated printer the print out of the method data
follows.
The measuring window is shown.
Insert / measure zero solution
The result will be displayed in two units of measurement
Insert / measure sample
