Qiagen QIAcube User Manual Download

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Operating Procedures

5-36

QIAcube User Manual 06/2008

WARNING

Risk of personal injury and material damage

[W16]

Do not use damaged rotor adapters. The rotor adapters

are for single use only. Do not reuse the rotor adapters as

they can be damaged by the high

forces experienced in

the centrifuge.

Press “Start” to start the centrifuge.

Summary of Contents for QIAcube

Page 1: ...Version 1 1 June 2008 Sample Assay Technologies QIAcube User Manual...

Page 2: ...IAcube QIAprep QIAquick DNeasy EpiTect MinElute RNeasy QIAGEN Group DNA ExitusPlus AppliChem Gigasept Lysetol Mikrozid Sch lke Mayr GmbH DECON QUAT Veltek Associates Inc RNaseZap Ambion Inc 2007 2008...

Page 3: ...8 1 9 Translations of warnings and cautions 1 8 1 10 Symbols on the QIAcube 1 26 2 Introduction 2 1 2 1 About this user manual 2 1 2 2 General information 2 2 2 2 1 Technical assistance 2 2 2 2 2 Pol...

Page 4: ...ping a protocol 5 9 5 3 Setting up the worktable 5 9 5 3 1 Loading the reagent bottle rack 5 9 5 3 2 Loading the tip racks 5 11 5 3 3 Loading the rotor adapters 5 12 5 3 4 Loading the samples 5 16 5 3...

Page 5: ...ng the worktable 6 15 6 6 5 Cleaning the gripper unit 6 16 6 6 6 Installing the centrifuge rotor and buckets 6 17 6 6 7 Operating the centrifuge after cleaning 6 18 7 Troubleshooting 7 1 7 1 General i...

Page 6: ...l data A 1 Environmental conditions A 1 Waste Electrical and Electronic Equipment WEEE A 3 FCC declaration A 4 EC declaration of conformity A 6 Appendix B B 1 Loading the centrifuge and shaker B 1 App...

Page 7: ...iven in a box like this one CAUTION The term CAUTION is used to inform you about situations that could result in damage to the instrument or other equipment Details about these circumstances are given...

Page 8: ...o the instrument C1 Avoid spilling water or chemicals onto the QIAcube Damage caused by water or chemical spillage will void your warranty In case of emergency switch off the QIAcube at the power swit...

Page 9: ...o a line power outlet that has a protective conductor earth ground Do not adjust or replace internal parts of the instrument Do not operate the instrument with any covers or parts removed If liquid ha...

Page 10: ...arts The QIAcube must be located out of direct sunlight 1 4 Biological safety Specimens and reagents containing materials from humans should be treated as potentially infectious Use safe laboratory pr...

Page 11: ...sure that the surrounding workplace is safe and that the instrument operators are suitably trained and not exposed to hazardous levels of infectious agents as defined in the applicable Material Safety...

Page 12: ...must be in accordance with all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration United States of America ACGIH American Conference of...

Page 13: ...nstrument must be operated with the door closed If the door sensor is not functioning correctly contact QIAGEN Technical Services Centrifuge Make sure that the rotor and buckets are installed correctl...

Page 14: ...tion maintain a minimum clearance of 10 cm at the sides and rear of the QIAcube Slits and openings that ensure the ventilation of the QIAcube must not be covered 1 8 Heat hazard The QIAcube worktable...

Page 15: ...nstrument The QIAcube must only be operated by qualified personnel who have been appropriately trained Servicing of the QIAcube must only be performed by QIAGEN Field Service Specialists DE Verletzung...

Page 16: ...corporels ou mat riels ne pas soulever l instrument tout seul WARNING Risk of personal injury and material damage W3 Do not attempt to move the QIAcube during operation DE Verletzungsgefahr und Besch...

Page 17: ...l instrument dangereux Il est interdit d interrompre volontairement ce conducteur Pr sence de tensions mortelles dans l instrument Lorsque l instrument est reli au secteur les raccords peuvent tre sou...

Page 18: ...infectious agents W7 Some samples used with this instrument may contain infectious agents Handle such samples with the greatest of care and in accordance with the required safety regulations Always we...

Page 19: ...lichen Regelungen zur Gesundheit und Sicherheit auf nationaler regionaler und lokaler Ebene zu ber cksichtigen FR Echantillons contenant des agents infectieux Certains chantillons utilis s avec cet in...

Page 20: ...safety regulations and laws DE Gef hrliche Chemikalien Einige der in Verbindung mit diesem Ger t verwendeten Chemikalien sind gesundheitsgef hrdend oder k nnen nach Beendigung eines Protokoll Durchlau...

Page 21: ...COSHH L vacuation des vapeurs et d chets doit tre conforme tous r glements et dispositions l gales au plan national d partemental et local concernant la sant et la s curit WARNING Risk of fire W9 When...

Page 22: ...duire des fum es toxiques WARNING Moving parts W11 To avoid contact with moving parts during operation of the QIAcube the instrument must be operated with the door closed If the sensor is not function...

Page 23: ...ouvrir manuellement le couvercle de la centrifugeuse WARNING Risk of personal injury and material damage W13 Raise the centrifuge lid carefully The lid is heavy and may cause injury if it falls down...

Page 24: ...Avoid touching it when it is hot DE Hei e Oberfl che Der Sch ttler kann Temperaturen bis zu 70 C 158 F erreichen Vermeiden Sie es den Sch ttler zu ber hren solange er hei ist FR Surface br lante L agi...

Page 25: ...gezogen sein kann sie sich w hrend der Zentrifugation l sen FR Risque de dommages corporels et mat riels La vis du rotor doit tre proprement serr e l aide de la cl du rotor fournie avec le QIAcube Si...

Page 26: ...eln lassen sich nicht korrekt aus dem Rotor entnehmen was einen Protokollabbruch zur Folge h tte FR Risque de dommages corporels et mat riels Assurez vous d enlever les couvercles des colonnes de cent...

Page 27: ...f das Geh use des QIAcube ge ffnet werden Verletzungsgefahr und Besch digung des Ger tes Keine Pflege und Wartungsarbeiten durchf hren die nicht in diesem Handbuch beschrieben sind FR Risque d lectroc...

Page 28: ...nden innerhalb der Zentrifuge ist daher Vorsicht angebracht FR Risque de dommages corporels Si du plastique s est cass des morceaux pointus peuvent se trouver l int rieur de la centrifugeuse Soyez att...

Page 29: ...bgedeckt FR D terioration de l appareil Eviter de renverser de l eau ou des substances chimiques sur le QIAcube Tout dommage caus par de l eau ou des produits chimiques mettra fin la garantie CAUTION...

Page 30: ...eil CAUTION Damage to the instrument C4 The QIAcube must not be used if the centrifuge lid is broken or if the lid lock is damaged Make sure that the rotor is installed correctly and that all buckets...

Page 31: ...QIAcube DE Besch digung des Ger tes Es d rfen weder Bleiche noch s ure oder laugehaltige Reinigungs oder Scheuermittel benutzt werden FR D terioration de l appareil Ne pas utiliser d eau de Javel de...

Page 32: ...ers kann bis zu 70 C 158 C hei werden Agitateur FR Risque de br lures la temp rature de l agitateur peut atteindre jusqu 70 C 158 F Near the centrifuge near the robotic arm EN Mechanical hazard avoid...

Page 33: ...ef hrdung der Abfallbeh lter kann durch biologisch gef hrliches Material kontaminiert sein und muss mit Handschuhen angefasst werden A l int rieur du compartiment d chets FR Danger Biologique le compa...

Page 34: ...on the back of the instrument EN CB mark of the IECEE member states Plakette auf der R ckseite des Ger tes DE CB Zeichen der IECEE Mitgliedsstaaten Plaque l arri re de l appareil FR Marquage CB des Et...

Page 35: ...pour l Australie Identification du fournisseur N17965 Type plate on the back of the instrument EN RoHS mark for China the restriction of the use of certain hazardous substances in electrical and elect...

Page 36: ...al 06 2008 Symbol Location Language Description Type plate on the back of the instrument EN WEEE mark for Europe Plakette auf der R ckseite des Ger tes DE WEEE Zeichen f r Europa Plaque l arri re de l...

Page 37: ...er manual must be followed to ensure safe operation of the instrument and to maintain the instrument in a safe condition 2 1 About this user manual This user manual provides information about the QIAc...

Page 38: ...cientists as well as to the researchers at QIAGEN We therefore encourage you to contact us if you have any suggestions about product performance or new applications and techniques For technical assist...

Page 39: ...page covers the general level of competence and training necessary for transportation installation use maintenance and servicing of the QIAcube Task Personnel Training and experience Transportation N...

Page 40: ...Introduction 2 4 QIAcube User Manual 06 2008 Page left intentionally blank...

Page 41: ...A plasmid DNA viral nucleic acids or proteins plus DNA and RNA cleanup The user selects a protocol using the touchscreen and loads plasticware samples and reagents onto the QIAcube worktable The user...

Page 42: ...ated if required by the protocol 2 Each lysate is transferred to a spin column in a rotor adapter If the lysate needs to be homogenized or cleared it is first transferred to the middle position of the...

Page 43: ...General Description QIAcube User Manual 06 2008 3 3...

Page 44: ...the user to select and run protocols install or delete protocols save data files and operate the shaker and centrifuge individually Messages displayed in the touchscreen guide the user through workta...

Page 45: ...ed to gain access to the worktable During operation of the QIAcube the door must remain closed and should only be opened when instructed to do so by the software If the door is opened during a protoco...

Page 46: ...he startup screen appears The instrument then automatically performs initialization tests To conserve energy the QIAcube can be switched off when not in use To switch off the QIAcube press the switch...

Page 47: ...bital shaker enables fully automated lysis of up to 12 samples Shaker adapters are available for 2 ml microcentrifuge tubes and 2 ml screw cap tubes Sample tubes are placed into a rack that fits onto...

Page 48: ...unted before starting a protocol run even if fewer than 12 samples are to be processed For more information see Appendix B or the loading chart available at www qiagen com MyQIAcube The centrifuge can...

Page 49: ...damage W16 Do not use damaged rotor adapters The rotor adapters are for single use only Do not reuse the rotor adapters as they can be damaged by the high g forces experienced in the centrifuge Reagen...

Page 50: ...ube worktable Tips can be purchased in prefilled tip racks holding 200 l filter tips 1000 l filter tips or 1000 l wide bore filter tips Two tip racks can be placed on the QIAcube Note Filter tips desi...

Page 51: ...ning of the robotic gripper and pipetting system on the QIAcube worktable and also includes the optical and ultrasonic sensor Robotic gripper The robotic gripper transfers spin columns during sample p...

Page 52: ...sponds to the number of samples in the shaker and that both shaker and rotor are correctly loaded The optical sensor also checks the type of tips loaded on the worktable and whether there are sufficie...

Page 53: ...ons Use a level workbench that is large enough and strong enough to accommodate the QIAcube Refer to Appendix A for the weight and dimensions of the QIAcube Ensure that the workbench is dry clean and...

Page 54: ...ion NEMA recommends that the QIAcube be correctly grounded earthed The instrument is equipped with a 3 conductor AC power cord that when connected to an appropriate AC power outlet grounds earths the...

Page 55: ...he black foam protector lid and lift up the box When lifting the QIAcube slide your fingers under both sides of the workstation and keep your back straight After unpacking the QIAcube check that the f...

Page 56: ...IAcube accessories Remove the QIAcube User Manual power cord and the centrifuge rotor and buckets from the foam packing material on top of the QIAcube Remove the USB stick rotor key rotor nut Allen ke...

Page 57: ...tch is set to the off position 2 Check that the voltage rating on the label at the back of the QIAcube matches the voltage available at the installation site 3 Plug the power cord into the power cord...

Page 58: ...erneath rotor position 1 Line up position 1 of the rotor with the pin on the rotor shaft and carefully lower the rotor onto the shaft Install the rotor nut on top of the rotor and tighten using the ro...

Page 59: ...bes onto the shaker 4 Tighten the 2 retaining screws using the Allen key Note Make sure to use the correct adapter for the sample tubes as this helps to ensure optimal instrument performance Using an...

Page 60: ...he instrument door 6 Undo the rotor nut on top of the rotor using the rotor key and carefully lift the rotor off the rotor shaft 7 Close the door 8 Press Tools in the main menu 9 Select Maintenance by...

Page 61: ...the accessories into the drawer The following accessories should be packed in air cushion bags Rotor key Rotor nut USB stick Allan key Shaker rack plugs Shaker adapter 17 Place the QIAcube onto the pa...

Page 62: ...on Procedures 4 10 QIAcube User Manual 06 2008 19 Seal the outside edges of the carton with tape to protect against moisture Note Using the original package minimizes damage during transportation of t...

Page 63: ...strument C2 Only use QIAGEN spin columns and QIAGEN Spin Kits with the QIAcube Damage caused by use of other types of spin column or chemistries will void your warranty The door of the QIAcube must re...

Page 64: ...to the previous step without saving any changes Edit Enables the user to enter values for certain parameters e g elution volume Increases the displayed value Decreases the displayed value Default Enab...

Page 65: ...agents to be used in the protocol Refer to the handbook supplied with the kit and to the relevant protocol sheet for instructions 3 Close the instrument door 4 Switch on the QIAcube at the power switc...

Page 66: ...ugh the list until the required sample material is highlighted and then press Select 8 Depending on the protocol further options may be available e g standard protocol homogenization using a QIAshredd...

Page 67: ...s Start and proceed to step 12 To enter user defined values for certain parameters such as elution volume press Edit Note Not all protocols allow user defined values to be entered 10 Press i or j to h...

Page 68: ...Manual 06 2008 11 Change the elution volume by pressing or The default value is displayed in the Default button Press Save to save the changes The parameter that has been changed will then be displaye...

Page 69: ...stick into the USB port see Section 5 5 Press Start to start the protocol run 13 Open the worktable door 14 Follow the instructions in the protocol sheet and displayed in the touchscreen for loading t...

Page 70: ...checked to make sure it is correctly loaded The number of rotor adapters in the centrifuge is checked to make sure that it corresponds to the number of samples in the shaker and that the rotor is corr...

Page 71: ...another protocol or switch off the QIAcube 5 2 3 Stopping a protocol You can stop a QIAGEN protocol if there is an emergency by pressing Cancel To confirm that you want to stop the protocol run press...

Page 72: ...ted by the rack labeling strip Pour carefully to ensure that the buffer does not foam or contain large air bubbles Reagent volumes are checked at during the load check at the start of the protocol run...

Page 73: ...optical sensor uses to identify the tip rack during the load check The rack for 200 l filter tips has a notch at the left forward side the rack for 1000 l filter tips has a notch at the left rear sid...

Page 74: ...each rotor adapter as described in the corresponding protocol sheet Ensure the 1 5 ml microcentrifuge tubes and spin columns have been pushed firmly into the appropriate position WARNING Risk of pers...

Page 75: ...Rotor adapter is incorrectly loaded with a 1 5 ml microcentrifuge tube The lid of the tube is not pushed all the way down to the bottom of the slot of the rotor adapter and could break off during cent...

Page 76: ...entrifuge tube causing the protocol run to crash If the protocol requires the use of a column in the middle position e g QIAshredder spin column cut off the lid before loading the column WARNING Risk...

Page 77: ...screw a spin column adapter ring not supplied with the QIAcube cat no 990399 onto the column Mounting a spin column adapter ring Break off the bottom closure of the spin column and place the spin colu...

Page 78: ...MyQIAcube All centrifuge buckets must be mounted before starting a protocol run even if fewer than 12 samples are to be processed WARNING Risk of personal injury and material damage W16 Do not use dam...

Page 79: ...positions are numbered for ease of loading Place the loaded shaker rack onto the shaker when instructed to do so by the software Loading the shaker rack with samples tubes that have attached lids If u...

Page 80: ...t use 1 5 ml microcentrifuge tubes on the shaker Because 1 5 ml microcentrifuge tubes can cause filter tips to stick during sample transfer their use on the shaker can damage the pipetting system and...

Page 81: ...to open the tubes before placing them onto the worktable Lids of microcentrifuge tubes should be securely placed into the slots at positions A and C If 3 tubes are to be placed onto the QIAcube workt...

Page 82: ...irst save them to the USB stick 1 Create a folder named New_Protocols on the USB stick 2 Go to www qiagen com MyQIAcube and search for the required protocol 3 Select the protocol to be downloaded and...

Page 83: ...to the QIAcube When the protocols have been successfully transferred to the QIAcube press OK Installing new protocols customized QIAGEN protocols or customer specific protocols Customized QIAGEN proto...

Page 84: ...roll through the list until the correct kit name is highlighted then press Select 3 Select the starting material this step may not be required for all protocols by pressing i or j to scroll through th...

Page 85: ...the protocol 5 5 Saving data files Data files such as run reports log files error logs or statistics can be saved to the USB stick Note The QIAcube can store up to 9 log files and run reports After th...

Page 86: ...tistics or Save calibration by pressing i or j to scroll through the list until the correct data file is highlighted and then press Select To save all files select Save all files Select function Tools...

Page 87: ...option is available for automatically saving the run report to the USB stick at the end of each protocol run Select USB autosave by pressing i or j to scroll through the list until it is highlighted...

Page 88: ...trument serial number the number of installed protocols as well as the system s memory capacity maximum and currently available can be easily displayed 1 In the main menu press Tools 2 Select Service...

Page 89: ...press Tools 2 Select Service by pressing i or j to scroll through the list until it is highlighted and then press Select 3 Select Software versions by pressing i or j to scroll through the list until...

Page 90: ...2 Select Settings by pressing i or j to scroll through the list until it is highlighted and then press Select 3 Select Brightness by pressing i or j to scroll through the list until it is highlighted...

Page 91: ...7 Operating the shaker The shaker can be operated individually if the QIAcube is not performing a protocol run The shaker can be used to heat and shake samples simultaneously shake samples without hea...

Page 92: ...08 Select Back 2 Shaker 3 Data excha Select item Tools 1 Centrifuge 4 Settings 5 To select the time speed and temperature press Edit and the appropriate parameter Press Start Tools Shaker Start Back E...

Page 93: ...and can be set within the ranges given below The default value is displayed in the Default button Press Save to save the changes Time 10 1800 seconds in increments of 10 seconds Temperature ambient t...

Page 94: ...ividually if the QIAcube is not performing a protocol run WARNING Risk of personal injury and material damage W3 Do not attempt to move the QIAcube during operation 1 Switch on the QIAcube at the powe...

Page 95: ...Operating Procedures QIAcube User Manual 06 2008 5 33 5 To select the speed time and acceleration press Edit and the appropriate parameter...

Page 96: ...nges Time 1 10 1200 seconds in increments of 10 seconds Speed 1 1000 12 000 x g in increments of 100 x g Time 2 0 1200 seconds in increments of 10 seconds Speed 2 1000 12 000 x g in increments of 100...

Page 97: ...color Press Back to exit the edit mode Select Back 1 Time 1 2 Speed 1 3 Time 2 Select item Tools Centrifuge 8 If required load opened 1 5 ml microcentrifuge tubes and or QIAGEN spin columns into the...

Page 98: ...k of personal injury and material damage W16 Do not use damaged rotor adapters The rotor adapters are for single use only Do not reuse the rotor adapters as they can be damaged by the high g forces ex...

Page 99: ...necessary at least every 6 months Following these procedures ensures that the QIAcube is free of dust and liquid spills The cleaning agent is chosen according to the goal of the cleaning procedure the...

Page 100: ...RNase Decontamination Solution Ambion Inc cat no AM9780 can be used for cleaning surfaces and submerging worktable items centrifuge rotor and waste drawer Removal of nucleic acid contamination DNA Ex...

Page 101: ...Damage to the instrument C5 Do not use bleach solvents or reagents containing acids alkalis or abrasives to clean the QIAcube WARNING Risk of electric shock W21 Do not open any panels on the QIAcube...

Page 102: ...wanted samples and reagents from the worktable Discard them according to your local safety regulations 3 Replace the lids of the reagent bottles and close tightly Store the bottles according to the in...

Page 103: ...ened with water To gain access to the modules within the robotic arm Press Tools in the main menu Select Maintenance by pressing i or j to scroll through the list until it is highlighted and then pres...

Page 104: ...portant Do not use alcohol or alcohol based disinfectants to decontaminate the QIAcube door Important Take care that no liquid runs down the touchscreen Liquid may be drawn through the dust protection...

Page 105: ...kit handbook 4 Remove all disposable rotor adapters including tubes and spin columns from the buckets 5 Remove the buckets from the rotor Undo the rotor nut on top of the rotor using the rotor key an...

Page 106: ...dual agent can cause the buckets to jam 8 Apply a few drops of mineral oil Anti Corrosion Oil rotor cat no 9018543 on a soft lint free cloth and wipe the bucket mount and rotor claw A thin invisible o...

Page 107: ...ge lid with a soft lint free cloth moistened with cleaning agent Incubate as appropriate clean with water and wipe dry with paper towels 4 Check the centrifuge gasket for damage If the gasket is damag...

Page 108: ...rotor and hang the bucket on the rotor Check that all buckets are properly suspended and can swing freely Important All centrifuge buckets must be mounted before starting a run 6 4 2 Cleaning the ins...

Page 109: ...the load check the robotic arm will pick up a tip aspirate ethanol and move to the tube The tip will remain in place above the tube for 2 minutes The tip will be detached 11 After the protocol is comp...

Page 110: ...1 Press Tools in the main menu 2 Highlight Maintenance scroll through the list by pressing i or j and press Select 3 Highlight Open lid scroll through the list by pressing i or j and press Select 6 6...

Page 111: ...ckets to jam 8 Apply a few drops of mineral oil Anti Corrosion Oil rotor cat no 9018543 on a soft lint free cloth and wipe the bucket mount and rotor claw A thin invisible oil film should cover the bu...

Page 112: ...eezers Important Make sure to use lint free paper towels Important Make sure that all plastic particles are removed Important Make sure the gaskets remain in the proper positions 3 Clean the centrifug...

Page 113: ...gent and rinse with distilled water 2 Clean the shaker rack labware tray heating adapter and reagent bottle rack with cleaning agent Rinse thoroughly with distilled water and wipe dry with paper towel...

Page 114: ...hlight Cleaning position scroll through the list by pressing i or j and press Start 6 Follow the instructions displayed in the touchscreen You will be asked to remove the waste drawer and the labware...

Page 115: ...supplied with the QIAcube Make sure that the rotor is securely seated WARNING Risk of personal injury and material damage W17 The rotor nut must be securely tightened using the rotor key supplied with...

Page 116: ...arameters for a centrifuge test protocol Press Tools in the main menu Highlight Centrifuge scroll through the list by pressing i or j and press Select To select the speed time and acceleration press E...

Page 117: ...ance Procedures QIAcube User Manual 06 2008 6 19 No unusual sound during centrifugation If no unusual sound from loose plastic particles can be heard during centrifugation the next sample run can be s...

Page 118: ...Maintenance Procedures 6 20 QIAcube User Manual 06 2008 Page left intentionally blank...

Page 119: ...rror messages or warnings are displayed in the touchscreen if a problem occurs during operation Refer to the error codes listed in this section if an error message or warning appears If error messages...

Page 120: ...ck error invalid rotor loading Check that the rotor is symmetrically loaded Clean the optical sensor 3201 Load check error not enough buffer Check the volume of reagent in the reagent bottles Make sur...

Page 121: ...t bottle is not overfilled The maximum volume is 30 ml and is indicated by a calibration mark 3208 Load check error no sample tubes or invalid sample tubes detected Check that samples have been loaded...

Page 122: ...l error protocol not found Reload the protocol from the USB stick see Section 5 4 Switch off the QIAcube wait for a few minutes and switch it back on again If the error persists contact QIAGEN Technic...

Page 123: ...ew minutes and switch it back on again If the error persists contact QIAGEN Technical Services 407 Protocol error unable to save protocol list Delete protocols from the QIAcube that are no longer requ...

Page 124: ...ocol 417 Protocol error parameter type mismatch Contact QIAGEN Technical Services for a new protocol 418 Protocol error invalid protocol version Load the protocol again If the error persists contact Q...

Page 125: ...h off the QIAcube wait for a few minutes and switch it on again Insert the USB stick into the USB port If the error persists contact QIAGEN Technical Services 703 File system error unable to read data...

Page 126: ...a collision If the error persists contact QIAGEN Technical Services 53 Motor driver error overheat prewarning Make sure that the QIAcube is operating under the correct conditions see page 1 3 and App...

Page 127: ...l Services 500 502 Program not ready or in error state A protocol cannot be started if the previous instruction is not completed and the instrument is busy Switch off the QIAcube wait for a few minute...

Page 128: ...for a few minutes and switch it back on again If the error persists contact QIAGEN Technical Services 3102 Motion error invalid disposal position Switch off the QIAcube wait for a few minutes and swit...

Page 129: ...tion Switch off the QIAcube wait for a few minutes and switch it back on again If the error persists contact QIAGEN Technical Services 3109 Motion error homing limit reached no light barrier found Ide...

Page 130: ...is not stuck to the tip adapter or that a spin column or microcentrifuge tube is not stuck to the robotic gripper If an item is stuck reinitialize the QIAcube or contact QIAGEN Technical Services If...

Page 131: ...Appendix A Switch off the QIAcube and wait 10 min before switching it back on If the problem persists contact QIAGEN Technical Services 5129 Centrifuge error imbalance detected Make sure that the rot...

Page 132: ...are correctly placed in the reagent bottle rack Be sure to use the right plasticware Check the volumes in the sample tubes and tubes of accessory buffer s if applicable Do not exceed the recommended a...

Page 133: ...elevant kit handbook and continue sample processing manually 7 2 7 Centrifuge Opening the centrifuge lid in the event of a breakdown In case of power failure the centrifuge lid can be manually opened...

Page 134: ...instrument door 4 Carefully move the robotic arm to the right side of the worktable furthest away from the centrifuge lid 5 Remove the screw protection on top of the centrifuge lid 6 Using the rotor w...

Page 135: ...ntrifuge lid release cord 8 Grasp the disk and pull to access to the cord Pull the cord firmly to release the lid from the lock Pulling the centrifuge lid release cord 9 Manually raise the centrifuge...

Page 136: ...ar of the QIAcube Slits and openings that ensure the ventilation of the QIAcube must not be covered Slits and openings for ventilation must not be covered You should be able to detect an air flow at t...

Page 137: ...otor is loaded symmetrically Check that the rotor buckets and gasket are not damaged 5 If there are loose parts inside the centrifuge or on the rotor clean according to Section 6 6 Liquid spills in th...

Page 138: ...be displayed when the QIAcube is switched on If this does not happen check that the power cord is properly connected If the QIAcube beeper sounds when the QIAcube is switched on but the startup screen...

Page 139: ...reagent volumes make sure that both rack labeling strips are attached to the reagent bottle rack These strips ensure that the reagent bottle rack is positioned correctly on the worktable for liquid de...

Page 140: ...Troubleshooting 7 22 QIAcube User Manual 06 2008 Page left intentionally blank...

Page 141: ...re discarded into the waste drawer Door The main door at the front of the QIAcube When open it provides complete access to the worktable Error code A 3 or 4 digit number that indicates a particular er...

Page 142: ...cube worktable Robotic gripper A component of the QIAcube robotic arm that moves spin columns during sample processing Rotor adapter A disposable plastic adapter that fits into a centrifuge bucket and...

Page 143: ...0 240 V AC 50 60 Hz 650 VA Europe Mains supply voltage fluctuations are not to exceed 10 of nominal supply voltages Overvoltage category II Air temperature 18 to 28 C 64 4 to 82 4 F Relative humidity...

Page 144: ...Width 65 cm 25 6 in Height 57 cm 22 4 in Depth 62 cm 24 4 in Dimensions door open Width 65 cm 25 6 in Height 81 cm 32 in Depth 62 cm 24 4 in Mass QIAcube 71 5 kg 157 6 lb Accessories 3 kg 6 6 lb Centr...

Page 145: ...c equipment by users in the European Union The European Directive 2002 96 EC on WEEE requires proper disposal of electrical and electronic equipment when it reaches its end of life The crossed out whe...

Page 146: ...nformed of the following facts and circumstances This device complies with part 15 of the FCC Operation is subject to the following two conditions 1 This device may not cause harmful interference and...

Page 147: ...easures Reorient or relocate the receiving antenna Increase the separation between the equipment and receiver Connect the equipment into an outlet on a circuit different from that to which the receive...

Page 148: ...y We herewith declare under our sole responsibility that the product QIAcube cat nos 9001292 93 94 95 meets all applicable requirements of the following European Directives Low Voltage Directive LVD 2...

Page 149: ...1 Appendix B Loading the centrifuge and shaker If fewer than 12 samples are to be processed make sure to load the centrifuge and shaker correctly see pictures below and following pages One or 11 sampl...

Page 150: ...Appendix B B 2 QIAcube User Manual 06 2008 Centrifuge Shaker...

Page 151: ...Appendix B QIAcube User Manual 06 2008 B 3 Centrifuge Shaker...

Page 152: ...Appendix B B 4 QIAcube User Manual 06 2008 Page left intentionally blank...

Page 153: ...gent bottle racks 3 rack labeling strips 8 200 l filter tips 1024 1000 l filter tips 1024 1000 l filter tips wide bore 1024 30 ml reagent bottles 18 rotor adapters 240 rotor adapter holder 1 5 ml micr...

Page 154: ...rips for the QIAcube reagent bottle rack for use with RNeasy Mini Kits QIAamp DNA Mini Kits QIAquick Kits QIAprep Spin Miniprep Kit QIAamp MinElute Virus Spin Kit and Ni NTA Spin Kit 990391 Rack Label...

Page 155: ...cube User Manual 06 2008 C 3 Product Contents Cat no Sample Tubes CB 2 ml 1000 conical screw cap tubes without skirted base 2 ml for use with the QIAcube 990382 USB Stick USB Stick for use with the QI...

Page 156: ...Appendix C C 4 QIAcube User Manual 06 2008 Page left intentionally blank...

Page 157: ...or the duration of the original warranty period and in no case beyond the original expiration date of original warranty unless authorized in writing by an officer of the Company Read out devices inter...

Page 158: ...Appendix D D 2 QIAcube User Manual 06 2008 Page left intentionally blank...

Page 159: ...ing conditions 1 3 A 1 Operation 5 1 Optical sensor 3 12 P Packing 4 7 Pipetting system 3 11 Power cord socket 3 6 Power switch 3 6 Protocol deleting 5 22 installing 5 20 5 21 running 5 2 stopping 5 9...

Page 160: ...3 10 loading 5 11 Tip sensor 3 12 Touchscreen 3 4 changing brightness 5 26 Transport locks releasing 4 4 Transportation conditions A 1 Troubleshooting 7 1 centrifuge 7 15 load check errors 7 2 shaker...

Page 161: ......

Page 162: ...ng Orders 800 933 965 Fax 800 930 439 Technical 800 930 425 Ireland Orders 1800 555 049 Fax 1800 555 048 Technical 1800 555 061 Italy Orders 02 33430411 Fax 02 33430426 Technical 800 787980 Japan Tele...

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