Qiagen QIAcube, User Manual

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General Description
3-2 QIAcube User Manual 06/2008
3.1 QIAcube principle
Sample preparation using the QIAcube follows the same
steps as the manual procedure (i.e., lyse, bind, wash, and
elute). No change in purification chemistry is required as you
simply continue to use trusted QIAGEN spin-column kits.
1.
Samples are lysed in the orbital shaker, which can be
heated if required by the protocol.
2. Each lysate is transferred to a spin column in a rotor
adapter. If the lysate needs to be homogenized or
cleared, it is first transferred to the middle position of the
rotor adapter.
3. Nucleic acids or proteins bind to the silica membrane or
purification resin of the QIAGEN spin column and are
washed to remove contaminants.
4. The spin column is transferred to a microcentrifuge tube
for elution of purified nucleic acids or protein.
For an example workflow using a QIAGEN spin-column kit,
see the flowchart on the next page.