Qiagen QIAcube, User Manual, Page: 70 / 162

Operating Procedures
5-8 QIAcube User Manual 06/2008
„
Fill the reagent bottles and place the reagent bottle
rack onto the worktable. Be sure to remove the lids
from the reagent bottles before placing on the
worktable.
„
Load opened accessory-buffer tubes required by the
protocol in positions A, B, or C.
„
Place the loaded rotor adapters into the centrifuge
buckets.
„
Load the shaker rack with samples, and place onto
the shaker. Be sure to use the correct sample tubes
and the correct shaker adapter, as described in the
protocol sheet (see page 5-16).
15. Close the QIAcube door.
16. After you have set up the worktable, the QIAcube
performs a load check, which consists of the following
tasks:
„
The instrument is initialized.
„
The shaker is checked to make sure it is correctly
loaded.
„
The number of rotor adapters in the centrifuge is
checked to make sure that it corresponds to the
number of samples in the shaker and that the rotor is
correctly loaded.
„
The reagent bottle rack is checked to make sure that
it is correctly loaded and there is sufficient buffer in
the reagent bottles.
Note : The volume of accessory buffers in positions
A, B, or C on the worktable is not checked. Be sure
to fill the microcentrifuge tubes with the exact volume
of buffer required.
„
The filter-tips are checked to make sure that the
correct type has been loaded and that there are
sufficient filter-tips for the protocol run.
17. If the load check is unsuccessful, an error message will
be displayed in the touchscreen. Resolve the error so that
the protocol run can continue (see Section 7.2.1).
Note : When resolving a load check error, only move or
change the affected samples or consumables.