www.pall.com/biopharm
23
Figure 15
System Setup for Flushing
Procedure
1. Fill the feed tank with water (or attach feed line to water supply).
2. Direct the retentate and permeate lines to drain.
3. Open the retentate and permeate valve.
4. Adjust the pump to deliver a flow rate of 5 to 10 L/min/m
2
(0.5 to 1 L/min/ft
2
).
Do not exceed a feed pressure of 2 barg (30 psig).
5. Adjust the retentate valve to split flow 25% retentate, 75% permeate.
6. Readjust pump to give required retentate flow rate.
7. Pass 10 L/m
2
(1 L/ft
2
) to waste.
8. Stop the pump.
4.3
Sanitizing and Depyrogenating the Cassette and Assembly
The following agents and conditions are recommended for sanitizing the specific Pall Omega
membrane types listed:
Volume Required:
5 L/m
2
(0.5 L/ft
2
)
Sanitizing and Depyrogenating Solutions:
0.1 to 0.5 N NaOH at 40 to 45 °C
0.1 to 0.5 N NaOH + 200 to 400 ppm NaOCl at 40 to 45 °C
10 to 50 ppm NaOCl (pH 6 to 8) at 40 to 45 °C
For cassettes that may have been subjected to high levels of endotoxin, the use of an acid
solution may be required as an alternative or additional procedure to the caustic processing
step listed above:
0.1 N Acetic Acid (HAc) at 40 to 45 °C
Warning: Many solutions recommended for sanitizing and depyrogenation may be corrosive
and/or hazardous. Ensure proper safety procedures are followed while handling, mixing and
preparing these reagents.
Retentate
Permeate
Feed
Tank
Waste
Waste
Feed
Pump
Pp
Rp
Fp
