42
Rotate the condenser turret to engage the suitable optical element
for the objective in use in the light path.
Engage the objective to be used in the light path.
Place the specimen on the stage and bring the specimen into
focus by moving the objective up or down.
Adjust the field iris diaphragm so that its image circumscribes the
field of view.
Adjust the aperture iris diaphragm to enhance the contrast.
Engage the DIC slider in the light path.
Move the prism movement knob of the DIC slider to select the inter-
ference color that can provide the optimum contrast in accordance
with the specimen.
U-DICT :
The background interference color is continuously
variable from the gray sensitive color to purple sen-
sitive color.
U-DICTS :
U-DICTHC :
U-DICTHR :
} With sensitive color observation using the U-UCD8, engage the
UUCDTP530 1 plate (sensitive color plate) in the light path.
· Setting the background color to dark enables an observation
like darkfield observation.
· Setting the background color to gray provides observation with
high contrast and 3D feeling with the gray sensitive color with
which the sensitivity is highest.
· Setting the background color to gray allows very small change
in phase to be observed as a change in color.
· There is a directional characteristic with the detection sensitivity
because of the configuration of the DIC prism. As a result, the
contrast may sometimes be improved by rotating the specimen
on the stage.
For simultaneous observation with reflected fluorescence ob-
servation, refer to the instruction manual entitled “REFLECTED
FLUORESCENCE SYSTEM”.
1
2
3
4
5
6
7
The background interference color is con-
tinuously variable from black to light gray.
CAUTION
6
Observation Method
