33
2.
Insert the Fine Gauge Thermocouple into the chamber by threading the wire through one of the screw holes
on the chamber riser
3.
Secure the end of the Fine Gauge Thermocouple to the bottom of a 35mm or similar Petri dish using some
tape making sure to avoid covering the head of the probe (see Figure 16).
4.
Fill the Petri dish with distilled water to a level sufficient to fully immerse the Fine Gauge Thermocouple.
This is a Reference Well simulating experimental conditions. Place the lid on the Petri dish, and insert the
dish in the incubation chamber. You can also use SENSOR LID-
# to close the dish (see the “Tip” below).
Tip
►
It is recommended using
an
Okolab SENSOR LID (available for purchase) to secure the Fine Gauge
Thermocouple in place in the Reference Well during calibration or operation in Sample Feedback Mode (see
Figure 17 and Table 1).
5.
Go in Control Mode page as shown in Figure 13 and
enable “Cage Mode”
6.
Wait until the system reaches the steady state (temperature indicator status is green).
7.
When the Fine Gauge Thermocouple reads a stable temperature open the Calibration page as shown in
and press “Adjust” and wait until the temperature measured by the Fine Gauge Thermocouple is
stable. If the temperature measured by the Fine Gauge Thermocouple is now close to the set point, then the
Calibration is complete. If the temperature measured by the Fine Gauge Thermocouple is far from the set
point, repeat the steps above until the temperature measured by the Fine Gauge Thermocouple reaches the
set point temperature.
8.
You can remove the Fine Gauge Thermocouple from the Reference Well petri-dish inside the incubating
chamber.
