NuAire LabGard NU-581-400 Operation And Maintenance Manual Download Page 54

Page: 54 / 107

OM0302

Rev 2/December 2020

Page 54 of 107

7.5.2 Calibration/Service Menu

The Calibration/Service menu provides a list of sub-menu items to accomplish all service tasks. For airflow

calibration, only the first three sub-menu items are used in the calibration process.

7.5.2.1 Cabinet Type

The cabinet type can be verified in the control system and is factory set and shouldn’t require alteration.

The cabinet type default information controls unit of measure, setpoints and limits based on the type and

size of cabinet.

To verify, press Service, then Factory Setup to verify the current type of cabinet is correct. If selection or

change is required, Press the current model to begin the process. Press cabinet type desired, then Press model

desired and lastly Press model size.

Summary of Contents for LabGard NU-581-400

Page 1: ...inar Flow Biosafety Cabinet Models NU 581 400 500 600 Bench Console Operation and Maintenance Manual December 2020 Revision 2 Series B Manufactured By Nuaire Inc 2100 Fernbrook Lane Plymouth MN 55447...

Page 2: ...ction from particulate contaminants as prescribed in NSF ANSI 49 Please read this manual carefully to familiarize yourself with proper installation maintenance and operation of the cabinet Other refer...

Page 3: ...rawings are appended in the back flap of this manual along with any modifications and or additions to procedures as outlined in this manual A copy of the original factory test report is also appended...

Page 4: ...stallation Assessment Test 7 9 Cleanliness Classification Test for Pharmacy Application 7 10 Main Control Board Description and Replacement 7 11 Digital Airflow Sensor Description and Replacement Sect...

Page 5: ...mental controls as required in the field of biological research or Hazardous Drug chemical containment The LFBSC when used with proper technique is an effective primary engineering control in obtainin...

Page 6: ...ply while specific agents are used o which measures are to be taken in the case of an accident Repairs to the device must be carried out only by trained and authorized expert personnel Keep these oper...

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Page 8: ...0302 Rev 2 December 2020 Page 8 of 107 2 0 Models and Features The model NU 581 LabGard ES HD Class II Type A2 Laminar Flow Biosafety Cabinet is manufactured in four sizes 4 ft 1 2m 5 ft 1 5m 6 ft 1 8...

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Page 12: ...ed Shipments 4 1 1 Terms are factory unless stated otherwise Therefore it is important to check each shipment before acceptance 4 1 2 If there is visible damage the material can be accepted after the...

Page 13: ...accessible for maintenance purposes Do not position the cabinet to prevent access to the power cord The power cord plug serves as the disconnect and should remain readily accessible If the outlet is...

Page 14: ...lve stem to gain clearance for valve body rotation Add Loctite 242 furnished to the threads and rotate valve body until secure with the valve stem for handle on the left side 5 2 4 Gas Service Nuaire...

Page 15: ...ries per cabinet size reference Electrical Environmental Requirements It is recommended that power to the cabinet whether hardwired or plug connected be on its own branch circuit protected with a circ...

Page 16: ...installation assessment tests The testing methods and equipment required are specified on the factory inspection report included with this manual see insert in back cover NOTE IT IS RECOMMENDED THAT T...

Page 17: ...ith Control Center And Armrest Removed 86 7 8 2207 92 7 8 2359 79 3 8 2016 54 7 8 1394 33 838 86 7 8 2207 92 7 8 2359 79 3 8 2016 66 7 8 1699 33 838 86 7 8 2207 92 7 8 2359 79 3 8 2016 78 7 8 2003 33...

Page 18: ...y compensates for filter loading voltage variances and other environmental effects A second digital dual thermistor airflow sensor in the exhaust airstream monitors for inflow velocity Both downflow a...

Page 19: ...icon may also be accessed for additional user menus 6 1 3 Run Mode Any time the blower icon is selected the RUN MODE screen will appear The Run Mode screen will display airflow setpoints and warm up...

Page 20: ...nto a ring back cycle of once every 2 seconds Pressing the alarm silence icon will silence the audible alarm for 15 minutes then into a ring back cycle of once every 2 seconds Alarm Types The middle o...

Page 21: ...essed will provide a list of menu items for various TouchLink functions 6 1 6 1 Setpoint Limits Setpoint Limits This menu screen will indicate the Biosafety Cabinet airflow control setpoints Access is...

Page 22: ...ions A description of each timer function is provided below Pressing the time area on the menu will bring up an keypad for time entry for each function Timer Functions Purge Timer This timer controls...

Page 23: ...ressed to turn on the outlet If timer is zero the outlet will stay on until turned OFF UV Light Timer This timer controls how long the UV light will remain on after the UV light icon has been pressed...

Page 24: ...ll automatically turn on and off together on a programmed schedule Once into the auto timer menu select the desired day for the auto timer to function If multiple days are desired each day will be req...

Page 25: ...6 1 6 3 Historical Performance When press a historical performance text the system displays downflow and inflow history Downflow History press downflow history the system displays the week log data i...

Page 26: ...OM0302 Rev 2 December 2020 Page 26 of 107 Inflow History press downflow history the system displays the week log data in graphical form of Inflow versus days...

Page 27: ...ovides the ability to alter display features and set date time Display Background colors of White Green Blue and Gray can be selected Touch display tone audible feedback can turn on off Display bright...

Page 28: ...et the time and date displayed on the LCD screen Time displayed is real time and will not automatically adjust for day light saving time 6 1 6 6 Change User Password This menu item provides the abilit...

Page 29: ...OM0302 Rev 2 December 2020 Page 29 of 107 Set New Password Retype new password...

Page 30: ...e position The power window also incorporates a safety auto reverse feature to eliminate any pinch hazard Operating the power window is performed by pressing either the UP or DOWN window icon The trav...

Page 31: ...e evaluated by appropriate safety personnel 6 2 2 Minimize Penetration of Air Curtain The minimum number of items necessary should be placed into the cabinet to prevent overloading but the work should...

Page 32: ...his precaution is merely an extension of good aseptic technique as practiced on open bench tops The good laboratory practices designed to minimize creation and or release of aerosols to the environmen...

Page 33: ...in some installations an additional advantage is obtained from purification filtration of the room air circulated through the equipment Because of this characteristic contributing to the quality of th...

Page 34: ...e performed prior to removing the needle from the stopper of the vial e A non splash collection vessel should be available in the Biosafety cabinet to discard excess drug solutions 6 3 6 Terminal Purg...

Page 35: ...unctionality and general good housekeeping The information provided below is intended to aid the development of facility Standard Operating Procedures SOP s for cleaning the equipment It is strongly r...

Page 36: ...perforated metal diffuser screen from the underside of the supply HEPA filter and place on the cabinet work tray NOTE Depending on the model the diffuser screen is secured to the cabinet by 8 32 scre...

Page 37: ...ust be performed only by specially trained and authorized service personnel in accordance with applicable national safety regulations The formaldehyde is vaporized within the tightly sealed sample cha...

Page 38: ...is started by accessing the CABINET DECONTAMINATION on the calibration service menu AUTO DECON H2O2 CD The Auto Decon process requires the use of Front and Top Seal Plates Seal plates for BSC s are so...

Page 39: ...ic via the upper and lower fasteners Refer to BCD 12281 At this point the sliding window assembly can be removed 4 Remove exhaust sensor shrouds 5 Remove the diffuser screen some methods of manual dec...

Page 40: ...be wiped clean with a suitable tissue and water after each use 3 Gowns A protective garment must be worn The garment should be made of lint free low permeability fabric and must have a closed front l...

Page 41: ...during the entire decon cycle To validate two decon processes and associated cabinet set up procedure Nuaire utilized a Bioquell Clarus II H O and a Steris X10 The following process was performed by...

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Page 43: ...Edges of the front window sealed with a low residue tape 6 Tubing connected to the inlet and outlet of X10 7 Insertion of Hydrogen Peroxide H2O2 sterilant cup and desiccant cartridge to X10 8 Current...

Page 44: ...OM0302 Rev 2 December 2020 Page 44 of 107 Auto Decon Set Up Select AUTO DECON SET UP to enter the specific Decon cycle parameters Select DECON FULL CYCLE TIME...

Page 45: ...wer run time at each interval as required by the automated decon process used Decon blower duty cycle allows the cabinet blower to run at a lower speed when used during the decontamination process The...

Page 46: ...o Decon Run To initiate the auto decon run cycle select and enter AUTO DECON RUN To prevent unauthorized entry into the auto decon cycle the password must be entered The password would be the same as...

Page 47: ...e auto decon H2O2 CD cycle Once the cycle has started it is desirable to let the cycle run until finished The display will indicate what portion of the cycle it s in and the time remaining It is possi...

Page 48: ...ident a puncture do not need replacement until the efflux velocity cannot be maintained or the access inflow velocity cannot be maintained at 100 LFPM 51 m s min This may permit the average downflow v...

Page 49: ...a Rotate both of the prefilter release clamps 90 cleaner and place wipe in prefilter Install prefilter seal over the used prefilter 6b Pull up and out prefilter of its position and lay on its Side to...

Page 50: ...damaged i e torn replace it Nuaire part number A 3071 25 Proceed to install HEPA prefilter making sure filter is seated level onto the gasket NOTE Only use same type of HEPA prefilter to assure proper...

Page 51: ...s handles to remove the panel NOTE Screws are used in lieu of acorn nuts and lock washers The screws have O rings and should be replaced if damaged or badly deformed The interior of the cabinet is now...

Page 52: ...ons to the motor remove the HEPEX pressure plenum and unbolt the motor blower assembly from the roof of the cabinet 4 places Always inspect the rubber isolation motor mounts and replace those that are...

Page 53: ...e of fall if a window counterbalance experiences a fault is also required to assure proper window function see Sliding Window Retention Verification in the Inspection Report 7 5 Airflow Control System...

Page 54: ...pe The cabinet type can be verified in the control system and is factory set and shouldn t require alteration The cabinet type default information controls unit of measure setpoints and limits based o...

Page 55: ...ress SET MOTOR TYPE to verify correct setting Upon a MASTER RESET the motor type is defaulted to DC ECM Single The NU 581 Series 1 115Vac requires the motor type selected to be DC ECM Single Always ve...

Page 56: ...setpoint establishes the airflow values that are to be maintained The high low limits establish the alarm boundaries from the nominal setpoint The default values have been established based upon the p...

Page 57: ...while the choke adjusts or balances the exhaust airflow as well as makes up for filter resistance tolerances Since it has been Nuaire s experience that the filters may not load evenly both adjustment...

Page 58: ...one minute or until downflow readings are steady Step 3 Place a velometer in the cabinet workzone on the horizontal plane 4 inches 102mm above the bottom edge of the window Spot check several points...

Page 59: ...S RELATED TO THE AIRFLOW MONITOR SENSOR CALIBRATIONS Step 1 Access sensor setup menu Step 2 Now measure the inflow velocity using the recommended procedure found in Table 7 0 If necessary adjust the e...

Page 60: ...desired a spot check in the downflow velocity may be performed if felt necessary Once entire cabinet has been balanced tighten liquid tight fasteners around choke adjustment shaft 7 5 3 3 Alarm Verif...

Page 61: ...9 19 620 498 19 380 492 Number of Readings Average Velocity ft min m s d Acceptance Criteria 1 Average downflow velocity 55 to 65 fpm 28 to 33 m s 2 Individual readings must be within 20 or 16fpm 0 08...

Page 62: ...980 1168 50 178 1274 54 375 1381 600 4 102 8 158 207 12 316 313 16 474 481 20 632 524 24 790 630 28 948 735 33 106 841 37 264 946 41 422 1052 45 580 1158 49 738 1263 53 896 1369 58 054 1475 62 212 158...

Page 63: ...of hazardous drug residue or appropriate precautions are taken per facility Standard Operating Procedures SOP S NOTE Cabinet should be on and running throughout the filter replacement process Hazardou...

Page 64: ...th duct tape or other suitable means to prevent contaminated air from migrating into the exhaust efflux All exhaust blowers fans should be turned off during the check The exhaust sensor shroud can als...

Page 65: ...sed from one end of the cabinet to the other 1 inch 25mm just inside the view screen at a height 6 inches 152mm above the top of the access opening 3 Pass a smoke source along the edges of the entire...

Page 66: ...control test points using a voltmeter Step 2 Using the primary or secondary inflow test method lower the speed control voltage to reduce the inflow by 20 from the certified testing value Step 3 Verify...

Page 67: ...ic compounding manipulations At Rest Particle Count Test Take 5 test points in 1 minute intervals on a grid in a horizontal plane as measured approximately 6 inches 152mm above the worksurface The gri...

Page 68: ...1 4 INCH 5 X 20MM 5 X 20MM 5 X 20MM NU 581 400 15 AMPS 3 AMPS 2 2 AMPS 1 AMP NU 581 500 15 AMPS 3 AMPS 2 2 AMPS 1 AMP NU 581 600 15 AMPS 3 AMPS 2 2 AMPS 1 AMP 7 10 3 Main Control Board Replacement No...

Page 69: ...an error in operation Cabinet type motor type and calibration data will not be affected with this reset Master Reset Resets all calibration cabinet type motor type sensor data and options settings ba...

Page 70: ...2 Rev 2 December 2020 Page 70 of 107 Select desired function from menu Perform either reset function as selected below Once factory reset is complete return to Calibration Service menu to enter any op...

Page 71: ...main menu At this point the cabinet MUST be turned off to complete the process Either unplug the cabinet or use the power switch within the control center to turn off the cabinet Turn the cabinet back...

Page 72: ...any maintenance action The airflow sensors are removed by turning the locking ring counterclockwise and gently pulling the sensor away from the connector To reattach the airflow sensor turn sensor in...

Page 73: ...n main control bd Display indicates Remote Override Active Indicates that the remote override is activated preventing the usage of the cabinet Power Loss Alert Indicates a power interruption has occur...

Page 74: ...adjust display brightness screen Run Time Failure Indicates a temporary microprocessor issue Clean alarm a see it reoccurs check line voltage to control board replace control board Recertification Due...

Page 75: ...ing the Calibration Service menu item a password screen will appear The default password is 9876 Once the service password is entered the Calibration Service menu will appear To exit either press the...

Page 76: ...mode This means with no controls or alarms activated When the manual control is on the downflow and inflow displays will indicate nominal setpoints Airflow adjustments can be made in the manual mode...

Page 77: ...off the blower may be turned on and off without restriction Night Setback setup The night setback setup menu item provides an access to night setback option and night setback Calibration Night setback...

Page 78: ...setback calibration The night setback calibration is calibrated as a percentage of the nominal setpoint and should be adjusted during the certification process to the desired level The default for nig...

Page 79: ...ice The service setup menu allows a QUALIFIED TECHNICIAN to configure calibrate and obtain functional service data Each parameter submenu will be described as well as the display will indicate present...

Page 80: ...0302 Rev 2 December 2020 Page 80 of 107 UV Light Run Time UV light run time value Reset if replacing the UV lamp Blower Motor Run Time Blower Motor Run time or enter old value if replacing control boa...

Page 81: ...fication time period Filter Maintenance This parameter allows the service technician to set the filter status data used to predict filter life availability Filter status is based on maximum RPM minus...

Page 82: ...ry Setup Option Setup The option set up menu allows A QUALIFIED TECHNICIAN to configure several different optional parameters per the menu below Each parameter sub menu will be described as well as th...

Page 83: ...he designated alarm silence time i e default time is 15 minutes When the alarm silence function is off airflow alarms cannot be silenced The alarm silence key will only appear for window high alarms t...

Page 84: ...This parameter allows for the selection of time to determine how many continuous seconds of an alarm condition occurs before activating an audible and visual alarm The time is displayed in seconds wi...

Page 85: ...terlock is on the window must be closed for the UV light to operate When the window interlock is off the UV light can be turned on regardless of the window position NOTE In addition to the TOUCHLINK s...

Page 86: ...e to run when the window is closed o Fan Relay Interlock This parameter allows for the selection of the fan relay interlock operation When the fans relay interlock is on and the blower switch is pendi...

Page 87: ...blower When the blower FL light interlock is on the LED light operation will be interlocked to the blower When the blower FL light interlock is off the LED light can be turned on at any time o Blower...

Page 88: ...indow closure Once the sliding window is closed the LED light will be disabled and the airflow will be reduced to a percentage of the normal setpoint NOTE Blower window interlock must be turned off to...

Page 89: ...be identical to the fan relay When the AUX relay function is off the AUX relay function provides delay On Off option o Accessory Outlet This parameter allows for the selection of the power connection...

Page 90: ...display menu to indicate the power window up and down icons The power window option functions using a 24 Vdc motor and internal feedback encoder to determine window position The power window also inco...

Page 91: ...nostics The General input and output diagnostics menu allows A QUALIFIED TECHNICIAN to exercise the control system s inputs and outputs Blower Sensor Diagnostic Blower sensor diagnostics reports the c...

Page 92: ...n points or move into desired access opening height default window height is 10 inches 254mm Then the Auto Cal can be used to lock in the window position based on the activation points of the window h...

Page 93: ...3mm past switch deactivation Bottom Middle Window Low Alarm Continues to travel up for 1 7 8 48mm past switch deactivation Used only with power window option Bottom Window Closed Stops when deactivat...

Page 94: ...and then remove This action will cause the cabinet to go into a downflow alarm condition The exhaust airflow reading should increase during this test However the downflow reading should go down to zer...

Page 95: ...and normally closed contact closure outputs which are activated whenever the bower is turned on However the AUX relay does have some conditional logic programmed The relay will activate whenever the...

Page 96: ...located on the front panel The sliding window is interlocked to the ultraviolet light so when the sliding window is raised the ultraviolet light will turn off If operational time duration is known th...

Page 97: ...Strep hermolyticus alpha type 5 500 Staphylococcus aureus 6 600 Staphylococcus albus 5 720 Micrococcus sphaeroides 15 400 Sarcina lutea 26 400 Pseudomonas fluorescens 7 040 Escherichia coli 7 040 Pro...

Page 98: ...pital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply 11 5 Pollution Degree 2 0 Pollution degre...

Page 99: ...ll Stainless Steel Worksurface Stainless Steel Window Faring Stainless Steel Window Glides HDPE Window Safety Glass Window Frame Stainless Steel Front Service Panel Painted Steel Front Decorative Pane...

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