41
VARIOUS OBSERVATION METHODS
5
5.2.3. Settings for phase contrast observation
2
1
A
Illustration 028 A-B: Settings for phase contrast: Inserting the appropri-
ate objectives.
All settings, including adjustment of
the lighting according to Koehler,
were made in bright field.
Turn the coarse focusing knob (1) to lower
the cross stage (2) completely.
3
10x
100x/Oil
10xPh
4
B
Now replace the objectives with the phase
contrast objectives (3) on the nosepiece (4).
Place the 20x objective in the free
space on the nosepiece that is not
assigned to any objective.
2
5
1
6
C
Illustration 029 C-D: Settings for phase contrast: Removing the place-
holder on condenser.
Turn the coarse focusing knob (1) to raise
the cross stage (2) to its upper limit. Then
turn condenser up-down knob (5) to lower
the condenser (6).
7
8
D
Slide the placeholder (7) out of the corre-
sponding holder (8) on the left-hand side of
the condenser.
Summary of Contents for NE620T
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