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Turning on the system:

1) Turn on the main switch (A). 

2) Turn on the computer (B).

3) If you need the mercury lamp, 

turn on the switch (C) and then 
press ignition (D).

4) Log onto the computer through 

“MIC users” and start the 

“NIS-Elements” software.

Turning off the system:

1) If you are the last user of the day, or if there is 

more than 30 min interval before the next user is 
booked, turn off the mercury lamp (C).

2) Close the software and “turn off” the computer 

through the start menu.

3) Turn off the main switch (A).

4) Fill out the logbook.

Nikon TE2000 fluorescent microscope

A

B

C

D

Summary of Contents for Elcipse TE2000

Page 1: ...hrough MIC users and start the NIS Elements software Turning off the system 1 If you are the last user of the day or if there is more than 30 min interval before the next user is booked turn off the mercury lamp C 2 Close the software and turn off the computer through the start menu 3 Turn off the main switch A 4 Fill out the logbook Nikon TE2000 fluorescent microscope A B C D ...

Page 2: ...ght path control If you are looking at your sample A taking fluorescence DIC images B or taking color images C A B C Control over the halogen light The main switch on the halogen power supply need to be on Regulate the intensity here Switching between objectives Choose the right objective for your sample ...

Page 3: ...Which camera do I choose Nikon DS U1 color camera or MuTech MV 1500 B W camera Turn on main switch before use ...

Page 4: ...he diaphragm A so that you can see the edges 2 Bring the edges of the field iris onto focus by raising or lowering the condensor focusing knob B 3 Senter the image of the field iris by using the condensor sentering knobs C 4 Open the diaphragm so the edges lie just beyond the field of view 1 2 3 4 or ...

Page 5: ... dishes MC 3 0 55 HMC LWD 40X MC 2 0 40 HMC LWD 20X Ph2 3 7 2 7 0 60 CFI Plan Fluor ELWD 40X Ph1 0 40 ELWD 20X Ph 1 16 0 0 30 CFI Plan Fluor 10X 17 1 0 13 CFI Plan Fluor 4X Remarks WD mm NA Description 6 5 4 3 2 1 Possit ion Correction collar You have to adjust the ring to the coverslip petridish thickness Turn until the image looks the sharpest Indicates the phase ring Use Ph2 for this objective ...

Page 6: ...2 for this objective Indicates the NA The higher the number the better the resolution Collar not corrected Collar corrected Use your fingers to turn the collar slightly Then adjust the focus Now keep turning the collar until you get the best sharp focus This is very important for the quality of your image Adjusting the correction collar on the objective ...

Page 7: ...Elements software 2 If the stage joystick is not responding make sure the stage is in auto position and connect the stage through the software Devices manage devices scroll down to the Märzhauser and then click connect ...

Page 8: ...scence and DIC images chose capture multichannel image multichannel setup 2 Tag off the settings you need and click ok 3 You are now ready to start imaging Click the multichannel icon left side and remember to go from visual to camera mode Setting up a multichannel acquisition ...

Page 9: ...e outer limits 2 Go to acquire large scan and define the image size you need Make sure you are located at the upper left of your sample before you start scanning 3 Position the first frame in the top left corner 3 x 2 will give you a large image as seen under Acquiring a large image with automated stage 1 2 3 6 5 4 ...

Page 10: ...ing The stage has not been initialized because it was on manual when the software was opened Make sure the stage is in auto position and connect the stage through the software Devices manage devices scroll down to the Märzhauser and then click connect ...

Page 11: ...Troubleshooting The camera setting is missing The previous user has probably closed the window Go to view controls camera settings ...

Page 12: ...Troubleshooting I can t find REX in order to save my data You need to connect to the server When choosing REX you will be asked for a password The password is ...

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