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5
VARIOUS OBSERVATION METHODS
5. VARIOUS OBSERVATION METHODS
As a continually upgradable microscope, NE620T can be extended on basic model to show
a variety of observation capabilities. The accessory sets are not included in the scope of
delivery and can be ordered separately.
Before using the various observation methods, it is recommended to set the
illumination according to Koehler analogous to the bright field adjustment see
chapter 4.5.
5.1. Dark field observation
5.1.1. Overview
Dark field microscopy
leads to a dark image background, in front of which the struc-
tures to be observed stand out brightly. As a result, it is possible to produce well-resolved,
high-contrast images of transparent objects with only very low contrast without the need for
prior staining of the specimen.
Typical examples for dark field microscopy are amoeba or epithelial cells, e.g. of the oral
mucosa. Fixing and subsequent coloring is not necessary. Dark field microscopy is also
particularly suitable for live cultures of aquatic organisms or algae, which one would like
to see in motion, which usually prohibits staining. In addition to the contrast gain achieved,
dark field observations are also aesthetically pleasing!
Spirogyra:
source: Nexcope
The method is primarily suitable for finding:
cell outlines, very fine structures like flagella,
very small particles, because they light up
brightly.
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