Nikon Wide Field Microscope Ee1078
Quick
start
Erasmus Optical Imaging Centre
document version 3
1
Start-up system
•
Switch on the power socket (#1)
•
Switch on Mercury lamp control unit
switch on and ignite (#2)
o
Wait ~10 seconds for the device to load
o
Hold ignite button until orange LED is active
•
Start the PC before other hardware is active
o
User account Nikon1078, no password required
•
Switch on the power socket (#3)
•
Switch devices on in the order of:
o
Halogen lamp
o
Incubator control-box
o
Microscope stand
located at the right, behind the serial plug
o
Stage controller
located at the back of the device
o
Shutter controller
•
Start imaging software Metamorph
o
Select "User" account
•
Upon error; drag error message from behind
Metamorph logo
o
Message:
ASI MS2000 not detected on COM1
o
Choose:
Retry
Shut down system
•
For live cell imaging:
o
Remove Tokai Hit incubator from the stage and place it on the table
o
Close the Tokai Hit incubator with a plastic well
•
Clean objective carefully
o
Remove oil with lens paper:
o
Hold lens paper tight between your fingers and wipe over objective
o
Wet your lens paper with 2-propanol and wipe 3-4 times more
•
Cover stage with lens paper cleaning box
•
If you are the last user of the day
•
Shut down all devices
o
Shut down Mercury lamp at last
o
Camera power is switched at the adapter box at the floor
•
Switch off the power socket located at the floor
