Nexcope NE620T, Manual

Page: 52 / 64

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5.4.5. Settings for reflected light fluorescent observation
PRESS AND
TURN
2
1
3
5
4
10x
A
Illustration 037: Settings for reflected light fluorescent microscopy.
Move On/Off switch (1) to position I to turn on the microscope.
BEFORE YOU START fluorescence observation, it is recommended to set the
microscope in bright field mode. This includes sample placement, adjustment of
the interpupillary distance, pre-focussing, diopter compensation and setting the
illumination according to Koehler.
Focus the specimen with 10x objective in bright field.
PUSH and TURN the brightness control knob (2) in the direction of the arrow to switch off
the transmitted light (3).
Turn the ON/OFF knob of the LED fluorescent attachment (4) in the direction of the arrow to
start the device and control the lighting.
Set the desired fluorescent module using the slider (5).
The following lighting control elements play a key role in fluorescent microscopy:
• field diaphragm, aperture diaphragm, condenser

Optimisation of contrast and light yield
By closing the aperture diaphragm on the condenser, about 90 % of the interfer-
ing fluorescent can be eliminated.
At the end of the observation, remove the LED fluorescent attachment so as not
to endanger other users.
5
VARIOUS OBSERVATION METHODS