6-4
This calibration method adopts five parameters:
0
R
,
a
,
b
,
c
and
d
.
This calibration method requires at least five calibrators. The concentration (or
activity) of calibrator 1 is 0, and the corresponding
R
is equal to
0
R
.
Parabola
Calibration formula:
c
bC
aC
R
+
+
=
2
This calibration method adopts three parameters:
a
,
b
and
c
.
This calibration method requires at least three calibrators. The calibration
parameters can be calculated through the method of polynomial least squares.
Spline
Calibration formula:
3
2
0
)
(
)
(
)
(
i
i
i
i
i
i
i
C
C
c
C
C
b
C
C
a
R
R
−
+
−
+
−
+
=
This calibration method requires 2 to 6 calibrators. The number of calibrators is set
to be n, so the calibration method has 4(n-1) parameters in total:
i
R
0
,
i
a
,
i
b
and
i
c
.
6.3 Calculating
Concentration
6.3.1 Calculating Concentration of Linearly Calibrated
Sample/control
One-point linear calibration
a
R
C
=
Where,
a
- calibration parameter
Two-point linear calibration
a
b
R
C
−
=
Where,
a
,
b
- calibration parameters
Summary of Contents for BA-88A
Page 1: ...BA 88A Semi auto Chemistry Analyzer Operation Manual...
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