46
Table 4. HVS Sequence Programming Voltages for Caliper NS12 A Chip
Reservoir
Load (V)
Inject (V)
Sample
A
-600
61
Buffer Waste
B
-1500
-100
Sample Waste
C
402
-11
Buffer
D
-792
-1500
Figure 32
. Example: Simple Sequence Wizard Electrokinetic Pinched Injection on a Chip.
Step A – Loading
7.
Click on the
Step B
tab to view its settings.
8.
Change the
Step B
name to “Injection.”
9.
Set the
Switch Step
setting to
Only Manually
.
10.
Set Channels A-D to regulate D.C. voltages, according to
11.
Table 4
.
You can either click and drag the
DC Voltage
slider to set the values, or enter the values directly in
the
DC Voltage
box. These settings are shown in
Figure 33
.
12.
Press
Apply
to upload the settings to the HVS.
13.
Choose
File > Save As
and name the file.
