Knauer BlueShadow Detector 10D User Manual Download Page 44

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HPLC · SMB · Osmometry

KNAUER

Wissenschaftliche Geräte GmbH

Hegauer Weg 38

D-14163 Berlin, Germany

Phone: +49 30 809727-0

Telefax: +49 30 8015010

E-Mail: [email protected]

Internet: www.knauer.net

www.knauer.net

See up-to-date manuals online:

www.knauer.net/downloads

KNAUER Wissenschaftliche Geräte GmbH

Technical data are subject to change without notice.

Please check our website for latest updates and changes.

Translation of the original German edition of this

manual, version 2.

Last manual update: 201

5-02-05

Printed in Germany on environmentally friendly paper

from sustainable forests.

BlueShadow are registered trademarks of:

KNAUER Wissenschaftliche Geräte GmbH

Summary of Contents for BlueShadow Detector 10D

Page 1: ...Detector 10D User Manual V7670 HPLC BlueShadow...

Page 2: ...quirements 15 Unpacking 15 Connectors on the Rear Side 16 Spring Strip for Remote Control 16 Plug Connector Assignments 16 Connecting Cables to the Pin Header 17 Analog Port 18 Ground 18 Initial Start...

Page 3: ...ring for the Device 32 What Maintenance Tasks can Users Perform on the Device 32 Cleaning the Flow Cell 33 Rinsing the Flow Cell 33 Cleaning the Lens of an Analytical Flow Cell 34 Cleaning the Light G...

Page 4: ...in analytical and preparative HPLC sys tem It is used in laboratories to analyze substance mixtures In an HPLC system the detector serves to detect substances in liq uids and show their concentration...

Page 5: ...min flow rates Automatical recognition and storing of device specific infor mation which are important for Good Laboratory Practice operation qualifications or repairing the device Operation Qualific...

Page 6: ...ufacturer Suitable solvents Acetic acid 10 50 at 25 C 77 0 F Acetone at 4 C 25 C 39 2 F 77 0 F 1 Acetonitrile Ammonia Benzene Carbon dioxide liquid 99 999 CO2 Chloroform Dilute ammonia solution Dilute...

Page 7: ...ersion 2 3 Not suitable solvents Bases Halogenated hydrocarbons e g Freon Mineral and organic acids except in buffer solutions Perfluorinated solvents e g Fluorinert FC 75 FC 40 Perfluorinated polyeth...

Page 8: ...ies made by KNAUER or a company authorized by KNAUER Delivery Detector with flow cell1 Power Cable Power unit Accessories kit UV Detector 10D User manual DE EN Installation Qualification Document DE E...

Page 9: ...ng safety glasses with side protection protective gloves overall What must be taken into account All safety instructions in the user manual The environmental installation and connection specifications...

Page 10: ...electrical shock or short circuit if liquids get into the device s interior For this reason place all bottles in a solvent tray Eluent lines Install capillaries and hoses in such a way that liquids ca...

Page 11: ...ble dangers related to the device are distinguished in per sonal and material damages in this user manual Type of damage Category Explanations Personal damages DANGER Lethal or very serious injuries c...

Page 12: ...High voltage hazard Electric shock hazard Electrostatic discharge hazard damages to system device or components can occur General warning sign moderate injuries can occur and also damages to system de...

Page 13: ...ts with a permission for use from your coun try In case of queries contact the Technical Support Power Plug Note Make sure that the power plug on the rear of the device is always accessible so that th...

Page 14: ...otice any damage contact the Technical Support and the forwarder company 2 Setup the delivery in such a way that the label is in the cor rect position Using the utility knife cut the adhesive tape Ope...

Page 15: ...via RS 232 Spring Strip for Remote Control CAUTION Zerst rung der Elektronik durch elektrostatische Entladung Geerdetes Armband tragen The spring strip is used for remote control The single ports tra...

Page 16: ...header Pull the power plug CAUTION Electrostatic discharge can destroy the electronics Wear protective bracelet against electrostatic dis charge and ground ERROR IN OUT IN After receiving a signal sh...

Page 17: ...0 V 000 nm Scaling 100 nm per Volt If 5 V voltage is applied the wavelength is 500 nm Ground Note The detector is not grounded if it is connected with a 24 V power supply to a 220 V outlet Voltages w...

Page 18: ...f insula tion from the ends of the cable 2 2 Using the depressor tool open the port Ground 1 on the spring strip 3 Insert the first cable end 4 Connect the second cable end to the port Ground on the r...

Page 19: ...fore be replaced by a flow cell before first usage Note The test cell is used during operation qualifications and must be stored Prerequisite Detector has been switched off Power plug is pulled out Pr...

Page 20: ...pacers on one or both sides and hence change the path length to 1 25 mm or 0 5 mm Prerequisite Flow cell has been disassembled Tool Allen wrench size 3 Procedure 1 Using the allen screwdriver unscrew...

Page 21: ...rew fittings 5 Nm for stainless steel fittings 0 5 Nm for PEEK fittings Note PEEK fittings withstand a maximum pressure of 50 bar Result Capillaries have been connected and the detector is ready for o...

Page 22: ...been assembled Procedure 1 Switch off the power supply 2 Plug the power supply into the device 3 Connect the power supply and the plug 4 Plug in the power supply 5 Using the power supply switch switc...

Page 23: ...and change the settings Note To avoid falsifying measuring values KNAUER recommends to press the Autozero button before every measurement and after exchanging eluents Control of the Device You can co...

Page 24: ...Analog In Zero point bl Analog In Scaling bm Lamp Operatin hours bn Serial number bo Firmware Fig 13 Menu structure CAL UVD V01 00 0 24626 243 HEAT LAMP D2 HEA LAMP D2 ON LAMP D2 OFF TC 0 5s Int Time...

Page 25: ...avelength but also indicates the status of the lamp HEA deuterium lamp lights up OFF Deuterium lamp is inactive ON Deuterium lamp is active Prerequisite Status display is active Steps Figure Procedure...

Page 26: ...stretch or compress the chromatogram You can choose the absorption value out of 3 values 0 01 AU 0 1 AU 1 AU Prerequisite Status display is active Process Figure Procedure 1 Keep left arrow key press...

Page 27: ...a scaling value Setting the Zero Point The zero point is predefined by the applied voltage Prerequisite Device has been connected to GROUND ANALOG port has been selected Process Figure Procedure 1 Ke...

Page 28: ...ption on the chromatography soft ware in the software manual There are two advantages to the use of software Continuous operation for triggered measurement is possible System status can be checked in...

Page 29: ...r cables longer than 2 m Prerequisite Detector is connected to a computer Status display is active Process Figure Procedure 1 Keep left arrow key pressed Press right arrow key until the cor rect displ...

Page 30: ...onality of the device Operation Qualification OQ Extensive test of the device s functionality A successfully exe cuted OQ ensures that the device functions properly Test Intervals Run the extensive fu...

Page 31: ...h a mild commercially available cleaning solution or with isopropanol What Maintenance Tasks can Users Perform on the Device Organic solvents are toxic above a certain concentration Ensure that work a...

Page 32: ...inse the flow cell to restore optimal sensitivity Note Dirty lenses or fiber optic connectors could falsify the measure ment Do not touch the lens or the fiber optic connector lends with bare hands We...

Page 33: ...part 3 3 The lens 2 is protected by a seal ring 1 This must be renewed every time the lens is disassembled 4 Remove the lens and clean with a clean soft cloth or with water in an ultrasonic bath 5 Aft...

Page 34: ...the holder and strip off the seal The seal ring needs to be renewed during every cleaning of the fiber optics 5 Clean the light guide with a clean soft cloth or with an appropriate solvent in an ultra...

Page 35: ...Do not touch the ends of the fiber optics with your fingers as this could falsify the measurement Handle the fiber optics with care avoid impacts or hard actions Move the fiber optics carefully withou...

Page 36: ...iber optics with your fingers as this could falsify the measurement Procedure Process Figure 1 Manually unscrew the fittings1 of the fiber optic from the flow cell Fig 27 Fitting of the fiber optics o...

Page 37: ...5 AU at 254 nm fiber optics version ASTM E1657 98 Drift 3 0 x 10 4 AU at 254 nm 4 0 x 10 4 AU at 254 nm fiber optics version ASTM E1657 98 Linearity 2 0 AU at 270 nm ASTM E1657 98 Time constants 0 00...

Page 38: ...ir humidity Below 90 non condensing General Power supply External input 100 240 V output 24 V DC 60 W Dimensions Width Height Depth 121 x 129 x 187 mm 121 x 129 x 236 with flow cell Weight 1 5 kg Prot...

Page 39: ...to the manufacturer at the time the damage occurs Wear parts fuses glass parts columns light sources cuvettes and other optical components Damage caused by negligence or improper operation of the devi...

Page 40: ...s and other oper ating materials All solvents and other operating materials must be collected sep arately and disposed of properly All wetted components of a device e g flow cells of detectors or pump...

Page 41: ...ont view 5 Rear view 5 16 Deuterium lamp Maximum operating hours 28 Status 26 Display A OUT 25 Control 25 Firmware 25 GLP lamp 25 Int Time 25 LAMP 25 Menu structure 25 Signal and reference value 25 SN...

Page 42: ...ontrol see spring strip 16 S Salts 7 Self calibration 23 Setup 24 Software 24 Local Area Network 30 Serial port 30 Solvents Flushing solution 33 Spare parts 9 Spring strip 16 ANALOG IN port 17 Error I...

Page 43: ...input current 16 A per phase EMC standard 2004 108 EC IEC 61010 1 2011 Safety requirements for electrical equipment for measurement control and laboratory use Low voltage directive 2006 95 EC IEC 613...

Page 44: ...KNAUER 2013 V7670A 0 02 10 13 Koe KNAUER Wissenschaftliche Ger te GmbH All rights reserved Technical data are subject to change without notice Please check our website for latest updates and changes T...

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