Knauer Azura ECD 2.1, Instructions Manual

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20 Installation
AZURA® Detector ECD 2.1 Instructions, V6685
of carbohydrates using anion-exchange chromatography with NaOH as
mobile phase). All gases except helium are removed completely. Helium
is not EC active and does not change the mobile phase properties signi-
ficantly. To prevent mobile phase contamination, only high-purity helium
must be used.
Installation & start-up
Please carefully follow the next steps for a successful installation and
start-up:
1. The installation of the flow cell and column is shown in Fig. 13.
2. If a manual injector is applied with position sensor, the sensor cable
must be connected to the VALVE connector on the rear panel to ena-
ble INJECT/LOAD functions. In the CONFIG screen the valve option
should be set to ‚Valve = present‘ manually.
3. An electrically actuated valve of VICI Valco (type E2CA, EHCA) can be
connected & controlled by the ECD 2.1. For that purpose a serial val-
ve cable is required. The serial cable should be connected to the VAL-
VE connector on the rear panel of the instrument to enable INJECT/
LOAD functions. This presence of this valve is automatically detected
and no further configuration has to be performed.
Fig. 13
Installation of flow cell and column in the ECD 2.1.
4.
Prior to connection of the HPLC system to the detector all metal parts
should preferably be passivated with 15% nitric acid during 20 min.
The acid is flushed through the pump, the pump tubing, the dampe-
ner, the injector (in load and inject position) and to waste.