Knauer Azura ECD 2.1, Instructions Manual

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17 Installation
AZURA® Detector ECD 2.1 Instructions, V6685
ClarityChrom® chromatography software
You find a detailed description on chromatography software in the corre-
sponding software instructions (document no. V1670) which can be found
on the KNAUER website: www.knauer.net/claritychrom
You find more information about supported software packages and firm-
ware versions in the list „KNAUER software device support“ (document
no. V1663)which can be found on the KNAUER website:
www.knauer.net/softwarecontrol
3.6 HPLC fluid connections
In this section the installation & priming of all relevant fluidic connections
are described to be able to use the ECD 2.1 for the analysis of substances
with (U)HPLC-ECD 2.1. When working with HPLC solutions and mobile
phases take the following precautions:
Use proper eye and skin protection when working with solvents. The
solvents used may be flammable, toxic or corrosive. Organic solvents
are toxic above a certain concentration. Ensure that work areas are
always well-ventilated! Use of open fire in the vicinity of this system
must be strictly prohibited. Do not install the system in the same
room with any other equipment that emits or could potentially emit
sparks. Wear protective gloves, safety glasses and other relevant
protective clothing when working on the device!
With respect to third-party (U)HPLC equipment, such as LC pumps, auto
samplers, injection valves, column heaters etc. used in combination with
the ECD 2.1 detector: The equipment connected to the system should
be specifically designed for use in (Ultra) High Performance Liquid Chro-
matography and capable of delivering flow rates typically in the range
between 1 µL/min up to 5 mL/min.
The manufacturer will not accept any liability for damage, direct or
indirect, caused by connecting this instrument to devices that do not
meet the relevant safety standards.
Tubing connectors
For optimal operation it is of the utmost importance that all tubing con-
nections on the injection valve, columns and flow cells of the injection
valves are made without introducing dead volumes to minimize peak
broadening, carry-over etc.
Use only the original polymeric fingertights supplied by the manu-
facturer with the purchased flow cell to make LC connections on the
flow cells inlet and outlet. Do not use metal tubing on the flow cell
because it may lead to damage or incorrect operation of the flow
cell. Use PEEK, PEEKsil or Fused Silica tubing (with FEP sleeves).