IBS MediaClave, User Manual

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MediaClave overview
INTEGRA Biosciences
Page 16
3.2.2.4 Specific configuration in program mode AUTOCLAVE
Figure 3.2: Positioning of containers in the vessel - example
When autoclaving liquids, the flexible temperature probe must be put into a reference container with the
same amount of liquid as the one with the medium liquid. The heating water (coupling medium) must be
higher than the steel base plate to ensure an optimal heat transfer to the product inside the container.
Instead of flasks (as shown in the pictures) could be used test-tubes as well.
3.2.2.5 Closing the vessel lid
1. Turn the vessel lid clockwise until it locks into position (see figure 2.1)
2. Ensure the inlet and outlet caps on the access port and the filling ports are screwed finger-tight
3.2.2.6 Removing of autoclaved material
1. Once the AUTOCLAVE cycle is finished press END to open the safety cover
2. Using both grips, turn the vessel lid anticlockwise until it unlocks and is released
3. Open the vessel lid
4. Remove the autoclaved material from the vessel
NOTE
: The program mode AUTOCLAVE is suitable to sterilise small amounts of medium in single con-
tainers such as e.g. “Erlenmeyer flasks” or test-tubes.
ATTENTION
: These containers must be placed as shown in the figure below to ensure an error-free
operation.
NOTE:
If there is a difficulty in opening the vessel lid, slightly unscrew the adding port caps to release any
residual pressure may help. This little residual pressure is mostly generated by the natural vaporisation of
the hot media or water which is within the vessel. After releasing the residual pressure, the addition port
must be closed again before opening the vessel lid.
Product
Reference
Product probe PT100
Product
Product
Product
Reference