Instructions
4
Maintenance
4.1
Sterilization and Cleaning
The Heated Lid, Heated Plate and the Temperature Controller do not have to be sterile, because the
incubation chamber has no direct contact with the cells and the cell culture medium. If sterilization
is necessary for some reason, we recommend using isopropanol (70%) or common lab disinfection
solutions based on quaternary ammonium compounds (e.g. Barrycidal 36 or Pharmacidal).
All parts of the Heating System can be cleaned from the outside. We recommend using ultrapure
water for cleaning. Fingerprints on the Heated Lid can be removed using isopropanol (70%) or
lens/eyeglass cleaning wipes.
Note!
When cleaning the Heated Lid, be careful when wiping the inner surface of the lid. The electro-
conductive coating may be damaged. Also take care not to damage the glass plate of the Heated
Lid.
4.2
Influence of Surrounding Temperature and Airflow
The surrounding, ambient temperature affects the temperature inside the microscopy chambers. De-
vices, such as computers and camera controllers, can significantly heat up small rooms. In this case,
we recommend equilibrating the room temperature to the typical experimental conditions at least
2-3 hours before calibrating.
The airflow can enhance the effect of temperature changes in the vicinity of the incubation chamber.
In a case where the airflow (e.g., air conditioning, etc.) cannot be stopped, we recommend protecting
the microscope as much as possible from any strong airflow. Please contact us for support.
The use of an XL–Incubator and/or an objective heater minimizes those effects and helps significantly
to stabilize surrounding conditions.
Version (26th April 2017)
Heating System
27
