Instructions
3
Operating the Heating System
Before starting an experiment, check that the Heating System fits on your microscope stage, and that
your cell culture vessels are compatible to the Heating Insert. Connect all parts (Section
) and
perform a temperature adjustment as explained in Section
3.1
Installation and Connection of the Parts
The components of the Heating System are connected as shown in Figure
Figure 11: Installation of the components of the Heating System.
To set up the Heating System follow these steps:
1. Place the Temperature Controller next to the microscope and connect the power supply.
2. Insert the Heated Plate into the opening of the microscope stage.
3. Insert a Slide or Dish into the Heating Insert and put the assembly in the opening of the Heated
Plate
4. Place the Heated Lid onto the Heated Plate.
5. Connect the electrical cables of Heated Lid (Channel 1) and Heated Plate (Channel 2) to the
plugs on the back of the Temperature Controller.
To setup a computer communication to the TempControl Software, the USB cable must be connected
between Temperature Controller and computer.
To adjust the sample temperature, use the temperature sensor of the Temperature Adjustment Set and
plug it into the correspondent connector on the back of the Temperature Controller. The temperature
sensitive ending is placed inside the sample.
3.2
Sample Preparation
Check that the cell cultureware you intend to use fits the Insert of the Heating System.
Prepare the cells according to your protocol and place the culture vessel in the Heating Insert. The
Heating Insert can be removed from the Heating System even with the holder in place. Then put the
whole assembly in the opening of the Heated Plate and close the Heated Lid.
Version (26th April 2017)
Heating System
23
