Instructions
Note!
Before shipment, all controllers are run through an in–house calibration with the correspond-
ing Heated Lid and Heated Plate. Only run the Temperature Controller with the corresponding
Heated Lid and Heated Plate to ensure the correct calibration of the sensors.
To measure and calibrate the sample temperature, use the provided Temperature Adjustment Set.
The temperature of the heated components has to be adjusted in small steps. Follow this procedure
to adjust the sample temperature:
1. Connect the temperature sensor (thermocouple type K) to the plug on the Temperature Con-
troller’s back.
2. Fill the provided µ–Dish or µ–Slide (depending on what you want to use for your experiments)
with water. Volumes should resemble the amount you need for your experiments.
3. Put the loose end of the thermocouple through the hole in the lid of the µ–Dish/µ–Slide and
make sure it dips into the water.
4. Place the µ–Dish/µ–Slide in the Insert and put the holder on.
5. Place the Insert in the Heating System, close the Heated Lid.
6. Set all parameters to create your experimental conditions, such as room temperature, air con-
ditioning, airflow, illumination, microscope settings, etc. For 37°C sample temperature, we
recommend to start with set temperatures of 37°C for the Heated Plate and 42°C for the Heated
Lid.
7. After 30 minutes, check the temperature of the external sensor in the InCuControl software. If
the sample temperature is still too low, raise the set values for Heated Plate and Heated Lid for
0.5-1°C and wait until the sample temperature is stable again (at least 10-15 min).
8. When the sample temperature has reached the desired value, write down set values for Heated
Plate and Heated Lid. (As well as the Objective Heater – if used)
Note!
The temperature of the Heated Lid must be set to at least some degrees warmer than the tem-
perature of the Heated Plate!
26
Heating System
Version (26th April 2017)
