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ibidi Heating System, Instructions Manual

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ibidi Heating System Instructions Manual Download Page 14

Preface

This manual is your guide to using the Heating System for cell culture experiments on an optical
microscope. It instructs first-time users how to use the instrument, and serves as a reference for
experienced users.

Before using the Heating System, please read this instruction manual carefully, and make sure that
the contents are fully understood. This manual should be easily accessible to the operator at all times
during instrument operation. When not using the Heating System, keep this manual in a safe place.
If this manual gets lost, order a replacement from the company website.

Summary of Contents for Heating System

Page 1: ...Version 25 04 2017 Heating System Instructions ...

Page 2: ...Instructions 2 Heating System Version 26th April 2017 ...

Page 3: ...Insert for µ Dishes 22 3 Operating the Heating System 23 3 1 Installation and Connection of the Parts 23 3 2 Sample Preparation 23 3 3 Start the Heating System 24 3 4 Setting Temperatures in the Control Panel 24 3 5 Temperature Adjustment in the Sample 25 4 Maintenance 27 4 1 Sterilization and Cleaning 27 4 2 Influence of Surrounding Temperature and Airflow 27 5 Troubleshooting 28 5 1 Focus not St...

Page 4: ...the indicated actions WARNING A potentially hazardous situation which if not avoided could result in serious injury or even death CAUTION A potentially hazardous situation which if not avoided could result in minor or moderate injury It is also used to alert against damaging the equipment or the instrument NOTE Additional information to help achieve optimal instrument and assay performance This la...

Page 5: ... Temper ature Controller to high temperatures The glass plate of the Heated Lid can break due to a mechanical impact If so the shards can lead to injuries if handled Be aware that when the system is switched on 10 V AC voltage is applied to the underside of the glass on the Heated Lid and bottom of the Heated Plate Do not touch the underside or put it in contact with anything conductive This could...

Page 6: ...ment control and labo ratory use Part 1 General requirements IEC 61326 1 2006 EMC Electrical equipment for measure ment control and laboratory use EMC requirements General requirements IEC CISPR 11 2009 modified A1 2010 Industrial sci entific and medical equipment Radio frequency distur bance characteristics Limits and methods of measure ment 6 Heating System Version 26th April 2017 ...

Page 7: ...80 RH Humidity 80 RH up to 30 C Operating altitude max 2000 m atmospheric pressure 800 1060 hPa Dimensions of Heated Plate with Lid Width 125 5 mm Depth 85 5 mm Height 25 5 mm Weight 210 g Temperature Control Range Heated Lid Channel 1 Ambient temperature min 18 C to 46 C Heated Plate Channel 2 Ambient temperature min 18 C to 45 C Recommended Temperatures before Adjustment see Section3 5 Heated Li...

Page 8: ...ing software Modification of software Use of software on multiple workstations network terminals or by other methods 3 The contents of this manual are subject to change without notice for product improvement 4 This manual is considered complete and accurate at publication 5 This manual does not guarantee the validity of any patent rights or other rights 6 If an ibidi software program has failed ca...

Page 9: ... modification or misuse 4 Use of unauthorized tubing or fluidic connectors 5 Use of consumables disposables and parts not supplied by an authorized ibidi distribu tor 6 Corrosion due to the use of improper solvents samples or due to surrounding gases 7 Accidents beyond ibidi s control including natural disasters This warranty does not cover consumables such as cell culture chambers and dishes tube...

Page 10: ...tem under conditions that pose a risk of explosion implosion or the release of gases 8 Avoid strong magnetic fields and sources of high frequency The Heating System might not function properly when located near a strong magnetic field or high frequency source 9 Avoid vibrations from vacuum pumps centrifuges electric motors processing equipment and machine tools 10 Avoid dust and corrosive gas Do n...

Page 11: ...ame WARNING To avoid risk electric shock and fire operate the Heating System with the supplied external power supply and the supplied cables and plugs CAUTION Ensure that the power supply is easily accessible The Heating System must be installed in a manner that does not hinder access to the power supply Connect the Temperature Controller to a Notebook by using the supplied network cable only nece...

Page 12: ...ater ultrapure or common lab disinfection solutions that are based on quaternary ammonium compounds e g Barrycidal 36 or Pharmacidal CAUTION The use of ethanol or other types of organic solvents may remove the instrument s paint Transporting the Heating System First switch off the Temperature Controller and then disconnect the Heating Devices from the con troller Carry the devices carefully and av...

Page 13: ...Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments All of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Direc tive We will however continue to monitor the application of the RoHS Direc...

Page 14: ...erence for experienced users Before using the Heating System please read this instruction manual carefully and make sure that the contents are fully understood This manual should be easily accessible to the operator at all times during instrument operation When not using the Heating System keep this manual in a safe place If this manual gets lost order a replacement from the company website ...

Page 15: ...ass top of the Heated Lid and the Heated Plate are actively heat controlled by the Temperature Controller The modular concept of the Heating Inserts enables the use of different consumables ranging from 60 mm and 35 mm dishes to regular slides Combined with a Gas Mixer humidity CO2 enriched and or O2 reduced air can be flushed in which makes the incubation chamber a fully controlled incubator righ...

Page 16: ...on provides a brief overview including the nomenclature for later descriptions in this doc ument 2 1 Components of the Heating System The components of the Heating System are listed with a description and the short name that is used in this instruction manual Description Short name Drawing Temperature Controller Temperature Controller Heated Lid with electrical cable and D sub connector to connect...

Page 17: ...in Figure 3 The Heated Lid and Heated Plate fit on a microscope stage equipped with a universal mounting frame for multiwell plates They are connected to the Temperature Controller The Heating System can be combined with the Gas Mixer unit that provides CO2 and O2 optional control and a defined humidified atmosphere Detailed information on the Gas mixer unit is given in the Gas Mixer instructions ...

Page 18: ...annels In addition this controller can be run either as a stand alone system or PC controlled The display shows the measured I is and set S set temperatures of all the channels You can navigate through the menu by using the control buttons on the right The LEDs on the front indicate the status and feedback of the channels connection to the power supply and USB connection Figure 4 Control LEDs LED ...

Page 19: ... rear of the Temperature Controller Figure 5 The setup of the connections is shown in Section 11 The available connections are Power supply USB to connect to the computer 4 control channels External thermosensor Type KTY 81 On Off switch Figure 5 Rear view of the Temperature Controller Version 26th April 2017 Heating System 19 ...

Page 20: ...ated Lid 127 5 mm 85 5 mm provides excellent optical quality and also allows for the use of all standard microscopy techniques including differential interference contrast DIC Due to the height of the lid we recommend using condensers with a working distance of 26 mm The upper glass part of the Heated Lid is electrically heat controlled The electrical cable 1 5 m is connected to the Temperature Co...

Page 21: ...s have two functions that both use the force of the integrated magnets The first function is to hold the Slides or Dishes firmly into position so as to avoid displacement during microscope stage movements The second is to create a tight contact of the Slides or Dishes to the metallic insert which then maximizes the heat transfer and ensures the stable heat control of the sample The Heated Plate pa...

Page 22: ...snap the dishes into a fixed position This will leave an inclined gap between the holder and the insert as shown in Figure 10 but this is intended to keep the dishes in place Figure 10 Schematic Drawing of µ Dish 35mm high in the Heating Insert To ensure a tight fit the lid of the Insert is tilting when closed Warning Heated Plate Heated Lid and Heating Inserts are positioned by strong Neodym Magn...

Page 23: ...e 4 Place the Heated Lid onto the Heated Plate 5 Connect the electrical cables of Heated Lid Channel 1 and Heated Plate Channel 2 to the plugs on the back of the Temperature Controller To setup a computer communication to the TempControl Software the USB cable must be connected between Temperature Controller and computer To adjust the sample temperature use the temperature sensor of the Temperatur...

Page 24: ...s you ensure that you do not start the heat ing process with the wrong temperature settings It is now possible to set the temperatures for the individual channels Section 3 4 After this you can re connect the heated devices For the first operation before adjusting the temperature in the sample for your specific setup the following temperature settings are recommended Table 4 Recommended temperatur...

Page 25: ...ontrols the temperature of the actively heated components The temperature in the sample will result from the interaction of all heated parts Therefore the temperature at the position of the cells must be adjusted An adjustment is recommended before starting the first ex periment The temperature adjustment should be done for each chamber type in order to control temperatures with an absolute accura...

Page 26: ... µ Dish µ Slide and make sure it dips into the water 4 Place the µ Dish µ Slide in the Insert and put the holder on 5 Place the Insert in the Heating System close the Heated Lid 6 Set all parameters to create your experimental conditions such as room temperature air con ditioning airflow illumination microscope settings etc For 37 C sample temperature we recommend to start with set temperatures of...

Page 27: ...tro conductive coating may be damaged Also take care not to damage the glass plate of the Heated Lid 4 2 Influence of Surrounding Temperature and Airflow The surrounding ambient temperature affects the temperature inside the microscopy chambers De vices such as computers and camera controllers can significantly heat up small rooms In this case we recommend equilibrating the room temperature to the...

Page 28: ...Anti Evaporation Oil ibidi 50051 to decrease evaporation If you have an actively controlled humidifying device increase the set value for relative humidity 5 2 1 Silicone Oil Procedure Anti Evaporation Oil Covering the medium with sterile silicone oil prevents all evaporation effects and is compatible with cell culture Please do not use mineral oil as this can be harmful to your cultureware Equili...

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