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User Guide 

www.hygiena.com 

INS2051 Rev A 

Program Menu 

The Automated Thermal Block is pre-programmed with four lysis protocols: 

Gram positive

 – use with Gram-positive bacteria, including 

Staphylococcus

 and 

Listeria*

 

*Note: For Listeria 24E assays, use the “24E” program. For real-time Listeria assays, use the “RT Listeria” program. 

Gram negative

 – use with Gram-negative bacteria, including 

Salmonella

E. coli

Campylobacter

Vibrio

 and 

Cronobacter

 

24E

 – use with BAX System 24E assays for 

Listeria

 and 

L. monocytogenes

 

RT 

Listeria

 – use with BAX System real-time assays for 

Listeria

 and 

L. monocytogenes

 

Turning the Thermal Block On and Off 

ON 

Turn on the thermal block by toggling 

the on/off switch on the back panel of 

the unit. 

At start-up, the LCD automatically 

displays the following series of 

messages: 

The first screen shows the version of 

software currently installed on the 

thermal block.

 

The second screen shows the seq file 

version number and the checksum 

number for validation purposes. 

Note: A change in either of these values 

indicates a modification to the thermal 

block software. If this occurs, any 

validation previously performed on the 

instrument may no longer apply. 

The start-up sequence is complete 

when the LCD display shows the 

program menu. 

OFF 

After finishing a run, you can either 

select another program or turn off the 

power to the unit by toggling the on/off 

switch on the back panel of the unit. 

Hygiena Thermal 

Block v3.2 

seq file ver: 02 

    checksum: EE 

Gram Positive

Gram Negative

Summary of Contents for DUP-1000

Page 1: ...Model DUP 1000 User Guide...

Page 2: ...ts affiliates All other trademarks are the property of their holders FIELD OF USE The Automated Thermal Block is manufactured by Torrey Pines Scientific for use with the BAX System Please see BAX Syst...

Page 3: ...Front Panel Display 4 Program Menu 5 Turning the Thermal Block On and Off 5 Running the Gram Positive Program 6 Running the Gram Negative Program 8 Running the 24E Program 10 Running the RT Listeria P...

Page 4: ...e target temperature and instructs the module to heat or chill the plate as required The block automatically provides the sequential heating and cooling conditions for lysis protocols eliminating the...

Page 5: ...and proceed with appropriate caution Indicates the presence of a hot surface or other high temperature hazard and proceed with appropriate caution Indicates the presence of an electrical shock hazard...

Page 6: ...rmal block unit on a level dry laboratory workbench Allow at least 4 inches 10 cm on all sides of the unit for ventilation 2 Insert one end of the provided power cord into the power supply and plug th...

Page 7: ...display An audible beep sounds when the button is pressed 3 SELECT CONTINUE button sets the menu item in the LCD display as the selected program and is also used to confirm required actions An audible...

Page 8: ...mal block by toggling the on off switch on the back panel of the unit At start up the LCD automatically displays the following series of messages The first screen shows the version of software current...

Page 9: ...mples prompt appears place a rack of prepared samples into the metal tube holder Note See the BAX System User Guide for information on preparing samples Press the SELECT CONTINUE button 5 The LCD disp...

Page 10: ...e LCD display changes to Completed and the thermal block sounds an audible beep to signal that the program has been completed The timer continues to show any additional time that samples are held at 4...

Page 11: ...unit sounds two audible beeps and the blue ACTION NEEDED LED activates The LCD display changes to Load Samples 4 After the Load Samples prompt appears place a rack of prepared samples into the metal t...

Page 12: ...emoved Press the SELECT CONTINUE button Note A timer in the bottom right corner counts up from 0 00 to show any additional time that samples are held at 4 C 10 The LCD display changes to Completed and...

Page 13: ...ion Press the SELECT CONTINUE button 2 The unit beeps once and the LCD display changes as the thermal block begins equilibrating to 37 C Note Do not load samples into the thermal block until the load...

Page 14: ...ps once and the LCD display changes as the thermal block begins equilibrating to 55 C While the block is heating follow the instructions in the BAX System User Guide to add additional lysing reagents...

Page 15: ...he thermal block sounds two audible beeps to signal the change A timer in the bottom right corner counts up from 0 00 during the temperature change No user action is required 15 After samples have coo...

Page 16: ...dration 18 After the PCR tablets have been hydrated remove the rack of cluster tubes from the metal tube holder Press the SELECT CONTINUE button 19 The LCD display changes to 24E Finished and the ther...

Page 17: ...mples prompt appears place a rack of prepared samples into the metal tube holder Note See the BAX System User Guide for information on preparing samples Press the SELECT CONTINUE button 5 The LCD disp...

Page 18: ...0 The LCD display changes to Completed and the thermal block sounds an audible beep to signal that the program has been completed The timer continues to show any additional time that samples are held...

Page 19: ...rinse to prevent damage to the equipment Soak metal tube holders in 20 bleach solution for about 5 minutes then rinse thoroughly with water and allow to air dry An alternate cleaner designed to remove...

Page 20: ...value entered in the LCD display SELECT CONTINUE button decreases the value entered in the LCD display Pressing SCROLL and SELECT CONTINUE buttons at the same time sets the value in the LCD display a...

Page 21: ...g the on off switch on the back panel The LCD displays a message that Default Settings Are Now Loaded 6 Continue to hold the SELECT CONTINUE button until the LCD displays Set Point off about 15 second...

Page 22: ...ating process If needed use the SELECT CONTINUE button to decrease the value of the set point Note Be sure to wait for the temperature reading of the thermometer to equilibrate before proceeding 11 Pr...

Page 23: ...ured temperature If necessary the thermal block will automatically adjust the Plate Temperature to align with the Set Point 16 Press the SCROLL button to increase the value of the set point to 95 C Th...

Page 24: ...the SELECT CONTINUE button to decrease the value of the Measured temperature to the value displayed on the thermometer Note When entering the Measured temperature do not include decimal places 21 Pres...

Page 25: ...running press the SCROLL button to display the Abort menu 2 Press the SCROLL push button until the arrow is next to the Abort option then press the SELECT CONTINUE push button 3 The LCD display shows...

Page 26: ...ng any step in the program This fault indicator appears on the LCD display until the program completes or the program is aborted by the user Affected samples should not be used with the BAX System Rem...

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