14
Automated Thermal Block
Running the RT
Listeria
Program
Samples are heated to 55 °C for 30 minutes, then 95 °C for 10 minutes, then held at 4 °C for at
least 5 minutes or until removed.
1 Turn on the thermal block, then press the
SCROLL button until the arrow
is next to the
RT Listeria
option.
Press the SELECT/CONTINUE button.
2 The unit beeps once and the LCD display changes
as the thermal block begins equilibrating to 55 °C.
Note: Do not load samples into the thermal block
until the load prompt appears on the LCD display.
3 When the thermal block has reached 55 °C, the
unit sounds two audible beeps and the blue
ACTION NEEDED LED activates. The LCD
display changes to “Load Samples”.
4 After the “Load Samples” prompt appears, place a
rack of prepared samples into the metal tube
holder.
Note: See the BAX System User Guide for information
on preparing samples.
Press the SELECT/CONTINUE button.
5 The LCD display changes to “RTLis Lysis 55C”. A
timer in the bottom right corner counts down from
30:00 and shows the number of minutes remaining
in this step.
6 After lysis at 55 °C is complete, the LCD display
changes to “Heating to 95C”. A timer in the bottom
right corner counts up from 0:00 during the
temperature change. No user action is required.
7 After the thermal block reaches 95 °C, the LCD
display changes to “Denaturing @ 95C”. A timer in
the bottom right corner counts down from 10:00
and shows the number of minutes remaining in
this step. No user action is required.
RT Listeria
Gram Positive
Equilibr.@ 55C
RTLis
Load Samples
RTLis
RTLis Lysis 55C
RTLis 26:38
Heating to 95C
RTLis 2:41
Denaturing @95C
RTLis 8:02
Summary of Contents for DUP-1000
Page 1: ...Model DUP 1000 User Guide...