Horiba Scientific Quanta-phi F-3029, Operation Manual

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Quanta-
φ
rev. C (23 Apr 2010) Troubleshooting
5-2
Flowchart for generating Quanta-
φ correc-
tion-factor files
Step 1. Calibrate both Excitation and Emission Monochromators
Step 2. Place Calibrated Reflector in Sample Compartment at 45º
between excitation and emission using F-1933 Solid Sample Holder
Step 3. Set Excitation Monochromator to 0 nm (zero order) to emit white
light.
Step 4. Adjust excitation bandpass to be between (0.1 and 1 nm)
and or place a Neutral Density Filter on the Excitation Port.
Usually at least OD1 up to OD2
Step 5.Choose counts per second mode for the emission detector and
integration time of 0.1 s. Adjust emission mono to 3 nm. Scan from 350
nm to 800 nm for an R928 PMT.
Step 6. Evaluate peak intensity in counts per second (cps).
Step 7. Scan emission from 350 to 800 nm using counts mode (not counts
per second) and the integration time required to collect 10
6
counts in the
peak channel
Step 8. Remove reflector from sample compartment and connect
integrating sphere
Step 9. Place cuvette reflector in integrating sphere at 45º between
excitation and emission or powder holder in bottom of sphere (with top
fiber).
Use same bandpass on emission and scan from 350-800 nm using same
conditions as Step7.
Step 10. Evaluate peak intensity, increase integration time to achieve 10
5
or higher counts in the peak and scan from 350-800 nm. The excitation
bandpass may also be increased slightly to increase the intensity of the
white light (usually not beyond 3 nm).
Step 11. Execute Spherecorrect Macro using the Mcorrect file for the
emission detector and the Sample data from Step 7 and the Sphere data
from Step 10.
Is Peak >> or << than 10
6
>>10
6
<<10
6
Decrease neutral
attenuation and or
increase bandpass to
intensify the white light
from excitation mono
to be as near 10
6
cps
as possible without
being over.
Increase neutral
attenuation and or
decrease bandpass to
intensify the white light
from excitation mono to
be as near 10
6
cps as
possible without being
over.