HemoCue HbA1c 501, Operating Manual

Page: 5 / 264

3
3. PRINCIPLES OF OPERATION
The blood sample (4μL) is collected at the sampling area of the reagent pack, which
is then inserted into the cartridge, where the blood is instantly lysed releasing the
hemoglobin and the boronate resin binding the glycated hemoglobin.
The reagent pack containing the blood sample is inserted in HemoCue® HbA1c 501
Analyzer (in which the cartridge has been placed). The cartridge is automatically
rotated, placing the blood sample in the measuring zone. The total hemoglobin is
photometrically measured by the diffused reflectance of the optical sensor
composed of both a LED (Light Emitting Diode) and a PD (Photo Diode).
The assembled cartridge is rotated and the rinsing solution washes out non-
glycated hemoglobin from the blood sample, enabling photomectical measurement
of glycated hemoglobin.
The ratio of glycated hemoglobin and total hemoglobin is calculated.
HbA1c % = A x [ x 100 ] + B
Where ‘HbA1c’ and ‘Total Hemoglobin’ are the signals obtained from the HemoCue HbA1c 501
system, ‘A’ and ‘B’ are the slope and intercept factors to correct the value for the calibration
standard of NGSP.
* NGSP: National Glycohemogolbin Standardization Program
4. HemoCue® HbA1c 501 Analyzer CONTENTS
At delivery carefully inspect the product for any obvious physical damage.
If any damage is evident, please consult your local distributor.
HbA1c
Total Hemoglobin
+
Contents
1. Hemocue® HbA1c 501 Analyzer 2. Operating Manual 3. Exclusive Power Adapter
4. Fan Filters
5. Daily Check Cartridge 6. Monthly Check Cartridge
GB