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OSMOMAT 050 User Guide
Version 1.1 (2011-10-12)
6.4 Performing the Measurement
1. Press the START button.
The zero point is re-checked internally, indicated by "BUSY"- LED lighting up
(Fig. 6-2)
. After
approx. 10 seconds, the “INJECT”- LED and the LED on the lower measuring cell flash and
prompt to inject the sample
(Fig. 6-3)
.
2. Aspirate a sample volume of 150-250 µL
Make sure to fill the syringe without air bubbles.
3. Injecting the sample
Insert the cannula through the septum as far as it will go. It is impossible to puncture the
membrane during this procedure. Inject the sample into the measuring cell using three equal
cycles of approx. 50 µL
(Fig. 6-4)
. This push-injection increases the rinsing effect and creates
an overpressure which is the trigger impulse to start the measurement program of the
OSMOMAT 050. It must be prevented to inject air bubbles or foam. Therefore, the syringe
plunger should not be pushed all the way to the end.
After injection, remove the syringe and clean it using water.
Figure 6-4: Injection in three stages
Figure 6-2: Checking the zero point
Figure 6-3: Injection prompt
Summary of Contents for OSMOMAT 050
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