Gilson PIPETMAN P, Manual

Page: 9 / 84

9
ENGLISH
5) Eject the tip by pressing firmly on the tip-
ejector button.
General Guidelines for Good Pipetting
1) Make sure that you operate the push-button
slowly and smoothly.
2) When aspirating, keep the tip at a constant
depth below the surface of the liquid (refer
to the table).
Table - Immersion Depth & Wait Time
Model Immersion Wait Time
Depth (mm) (Seconds)
P2 1 1
P10 1 1
P20 2-3 1
P100 2-4 1
P200 2-4 1
P1000 2-4 2-3
P5000 3-6 4-5
P10ml 5-7 4-5
3) Change the tip before aspirating a different
liquid, sample, or reagent.
4) Change the tip if a droplet remains at the
end of the tip from the previous pipetting
operation.
5) Each new tip should be pre-rinsed with the
liquid to be pipetted.
6) Liquid should never enter the tip-holder; to
prevent this:
- press and release the push-button slowly
and smoothly,
- never turn the pipette upside down,
- never lay the pipette on its side when there
is liquid in the tip.
7) When pipetting liquids with temperatures
different to the ambient temperature, pre-
rinse the tip several times before use.
8) You may remove the tip-ejector (see “Main-
tenance”) to aspirate from very narrow tubes.