Gilson PIPETMAN P, Manual

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7
ENGLISH
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With a precise pipetting technique P2 may be
used to aspirate volumes down to 0.1 µL, P10
at 0.5 µL, and P200 at 30 µL.
Volatile Solvents
For volatile solvents you should saturate the
air-cushion of your pipette by aspirating and
dispensing the solvent repeatedly before
aspirating the sample.
6 - PIPETTING
1) Fit a new Gilson Diamond Tip.
Plastic tips are for a single application - they
must not be cleaned for reuse. Push the tip-
holder into the tip using a slight twisting
motion to ensure a firm and airtight seal.
(1) For P2 and P10: tip ejector extensions
are supplied.
(2) P5000 and P10ml: insert a filter into the
tip holder before fitting a tip. (If the filter
gets dirty it should be replaced with a
clean one.)
(3) P5000 and P10ml: these models are not
equipped with tip-ejectors.
2) Pre-rinse the tip.
Some liquids (e.g. protein-containing solu-
tions and organic solvents) can leave a film
of liquid on the inside the wall of the tip; pre-
rinse the tip to minimize any errors that may
be related to this phenomenon.
Pre-rinsing consists of aspirating the first
volume of liquid and then dispensing it back
into the same vessel (or to waste). Subsequent
volumes that you pipette will have levels of
accuracy and precision within specifications.
3) Aspirate.
Press the push-button to the first stop (this
corresponds to the set volume of liquid).
Hold the pipette vertically and immerse the tip
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