background image

Protection of your pipette and 

your work:

If step

 

 

is forgotten, the volume 

selected will be automatically 

locked during the next purge.

NOTICE

SE

T

TIN

G T
H

E V
O

LU

M

E

7

PIPETMAN L®

 

|

 

USER’S GUIDE

1

5

 

P2L

1.25 µL

2

0

7

5

 

P10L

7.5 µL

1

2

5

 

P20L

12.5 µL

 

P100L

0

7

5

75 µL

 

P200L

1

2

5

125 µL

 

P1000L

0

7

5

0.75 mL

 

P5000L

1

2

5

1.25 mL

 

P10mL L

0

7

5

7.5 mL

0

7

5

 

Lx10

7.5 µL

1

2

5

 

Lx20

12.5 µL

 

Lx200

1

2

5

125 µL

 

Lx300

1

2

5

125 µL

Single channel models

Multichannel

models

Chapter 2

SETTING THE VOLUME

This section applies to variable volume models only.

The volume of liquid to be aspirated is set using the 

volumeter. The dials are colored either black or red to 

indicate the position of the decimal point, depending 

on the model (refer to Figure 4).

MODEL

COLOR OF VOLUMETER NUMBERS

BLACK

RED

INCREMENT

P2L

µL

0.01 µL

0.002 µL

P10L to P20L – Lx10

µL

0.1 µL

0.02 µL

P100L – P200L – 

Lx200 – Lx300

µL

0.2 µL

P1000L

0.01 mL

mL

0.002 mL

P5000L

0.01 mL

mL

0.002 mL

P10mL L

mL

0.1 mL

0.02 mL

Lock System

For additional safety, the volume selected is lockable.

With the left or right thumb, unlock the thumbwheel 

by pushing it up.
The volume is set by turning the thumbwheel. 
The thumbwheel may be turned using only one hand 

to slowly reach the required setting.
Push down the thumbwheel; the new volume 

selected is locked.

To obtain maximum accuracy when setting the volume, 

proceed as follows:

 

When 

decreasing

 the volume setting, slowly reach 

the required setting, making sure not to overshoot 

the mark.

 

When 

increasing

 the volume setting, pass the 

required value by 1/3 of a turn and then slowly 

decrease to reach the volume, making sure not to 

overshoot the mark.

Figure 4

Dial Colors by Model

1

2

1

2

Figure 5

Volume unlock

Chapter 3

USER ADJUSTMENTS

Adjustment of the factory 

calibration must only be 

performed using a special 

calibration key.

NOTICE

This section applies to Fixed models only.
PIPETMAN L Fixed is factory calibrated using distilled 

water and very high precision balances. The nominal 

value of the PIPETMAN L Fixed may be slightly adjusted 

to compensate for liquids of different density or 

viscosity.
To accomodate for density or viscosity, an adjustment 

with one full turn of the calibration key in either 

direction equals:

± 0.05 µL  (models F1L to  F2L) 

± 0.2 µL  (models F5L to F10L)

± 0.5 µL  (models F20L to F25L)

± 2.0 µL  (models F50L to F100L)

± 5.1 µL 

(models F200L to F250L)

± 20 µL 

(models F300L to F1000L)

102 µL 

(model F5000L)

The cover is graduated in tenths of the key revolution 

(letters A, B,... J) and is read from left to right. Each 

small graduation mark represents 0.25 of the distance 

between each letter.

Figure 6

To adjust your PIPETMAN L Fixed, hold the key in 

the highest position to avoid banging it into the 

hook.

Summary of Contents for FA10001M

Page 1: ...Variable volume single and multichannel models and fixed volume models A UTOCLAVA B L E FULLY VARIABLE VOLUME MODELS User s Guide EN...

Page 2: ...t 13 MAINTENANCE FOR SINGLE AND FIXED MODELS 14 Change the Tip Ejector 14 Change the Tip Holder 14 Service the Piston Assembly 15 Change the Seals 16 MAINTENANCE FOR MULTICHANNEL MODELS 16 CLEANING AN...

Page 3: ...ures The Serial Number is engraved on the body of the pipette It provides unique identification of your pipette and the date of manufacture Example GB58672 The Bar Code on the box and the certi cate o...

Page 4: ...ejector Graduated cover Tip holder Personal label You can identify your pipette with a name tag 1 Pry out the window by inserting a small screwdriver in the access slot 2 Position the name tag in the...

Page 5: ...5 0 012 0 12 0 08 5 0 075 0 030 0 12 0 08 Max 10 0 100 0 040 0 12 0 08 P20L P N FA10003P OR FA10003M Min 2 0 10 0 03 0 20 0 10 10 0 10 0 05 0 20 0 10 Max 20 0 20 0 06 0 20 0 10 P100L P N FA10004P OR F...

Page 6: ...ctor the part number is FA10002P For the same pipette with a stainless steel tip ejector the part number is FA10002M NOTICE INTRODUCTION 6 INTRODUCTION PIPETMAN L FIXED MODELS MODEL VOL L GILSON ISO 8...

Page 7: ...bwheel the new volume selected is locked To obtain maximum accuracy when setting the volume proceed as follows When decreasing the volume setting slowly reach the required setting making sure not to o...

Page 8: ...AN L Fixed is readjusted to compensate for a particular solution the nature of the liquid can be noted on the labels inserted in the slot under the counter Figure 7 Adjust PIPETMAN L Fixed volume sett...

Page 9: ...our Tipacks and Towerpacks Ejector spacer for PIPETMAN L Multichannel 10 L According to the tip used D10 or DL10 you might have to exchange the ejector spacer the broad one is dedicated to D10 and the...

Page 10: ...along the inside surface of the vessel 4 Release the push button smoothly Eject the tip by pressing firmly on the tip ejector button For the multichannel models use a Gilson reagent reservoir refer t...

Page 11: ...C but the speci cations may vary according to the temperature refer to the ISO 8655 2 standard for conditions of use Chapter 5 ACCESSORIES To make pipetting more comfortable and more secure Gilson ha...

Page 12: ...nscrew tip holder Tighten connecting nut Connecting nut is loose Tighten connecting nut Pipette is not precise Tip holder is loose Tighten connecting nut Connecting nut is loose Tighten connecting nut...

Page 13: ...vel goes down there is a leak For the P1000L P5000L and P10mL L Single channel models for the F300L to F5000L Fixed models 1 Fit a PIPETMAN DIAMOND tip 2 For the variable volume models set the pipette...

Page 14: ...emove the tip ejector To refit 1 Push the ejection button 2 Slide the tip ejector along the tip holder 3 Clip the tip ejector on the body of the pipette Change the Tip Holder no tools required 1 Remov...

Page 15: ...miniaturized parts it is best not to disassemble these pipettes yourself 1 Remove the tip ejector refer to Change the Tip Ejector page 14 2 Unscrew the connecting nut by turning it clockwise 3 Carefu...

Page 16: ...he seal lubricate them lightly Please place them in the correct order 6 Reassemble the pipette refer to the gure 18 on page 15 7 Tighten the connecting nut turn counter clockwise 8 Re t the tip ejecto...

Page 17: ...seals must be degreased with isopropanol or ethanol before being immersed in another ultra sonic bath Small round brushes with soft plastic bristles may be used to clean the interior of the tip holder...

Page 18: ...t cloth or lint free tissue soaked with distilled water or sterile water Lower Part Volumetric module The following components only can be immersed in a decontaminant solution connecting nut tip eject...

Page 19: ...SCRIPTION P200L P1000L C D E Service Kit 1st level FA07004 FA07005 A B F Service Kit 2nd level FA07305 FA07306 C D Seal guide O ring 5 sets FA07015 FA07016 P5000L FA10007 AND P10ML L FA10008 LABEL DES...

Page 20: ...F507003 H3 H4 Ejector spacer D10 F807114 F807115 P8X20L FA10009 AND P12X20L FA10010 LABEL DESCRIPTION 8X20 12X20 F3 F4 Tip ejector F507005 F507006 G Ejector lock F507008 F507008 H1 H2 Ejector spacer F...

Page 21: ...F100L FA10024 LABEL DESCRIPTION F20L F25L F50L F100L C D E Service Kit 1st level FA07002 FA07003 A B F1 Service Kit 2nd level FA07069 FA07070 C D Seal guide O ring 5 sets FA07013 FA07014 F200L FA1002...

Page 22: ...void in the event pipettes are altered or modified by any party other than Gilson or its designates Gilson s sole liability under this warranty shall be limited to at Gilson s sole option repair or re...

Page 23: ...Notes 23 PIPETMAN L USER S GUIDE NOTES...

Page 24: ...USA T 608 836 1551 or 800 445 7661 F 608 831 4451 Gilson S A S 19 avenue des Entrepreneurs BP 145 F 95400 Villiers le Bel France T 33 0 1 34 29 50 00 F 33 0 1 34 29 50 20 LT801575 B 08 2017 Gilson Inc...

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