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18
CLEANING AND DECONTAMINATION
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PIPETMAN L®
2. Procedure
PIPETMAN L variable volume models can be sterilized by steam autoclaving at 121°C (252°F), 1 bar relative
pressure for 20 minutes without disassembly.
When autoclaving, the pipette will dry better and faster without using a bag.
3. After autoclaving
Following the autoclaving cycle, leave the pipette to cool down to room temperature and dry completely
before use.
Checking should be carried out according to your procedure.
FOR FIXED MODELS
The upper part (body) and the piston assembly of the pipette are
not
autoclavable.
Only
the following
parts may be autoclaved: tip ejector, tip holder and connecting nut.
Chemical Decontamination
You may choose to decontaminate your pipette chemically, in accordance with your own procedures.
Whatever decontaminant you use, check with the supplier of the decontaminant that it is compatible
with stainless steel and the plastics used in the construction of the pipette: PA (Polyamide), PBT
(Polybutylene Terephtalate), PC (Polycarbonate), PC/PBT (Polycarbonate/ Polybutylene Terephtalate),
POM (Polyoxymethylene), PVDF (Polyvinylidene Fluoride), PP (Polypropylene), PPA (Polyphthalamide), or
PPS (Polyphenylene Sulfide).
FOR VARIABLE VOLUME SINGLE AND FIXED MODELS
Upper Part (Handle)
1.
Wipe the upper part (handle) of the pipette with a soft-cloth or lint-free tissue soaked with the
chosen decontaminant.
2. Wipe the upper part of the pipette with a soft- cloth or lint-free tissue soaked with distilled water or
sterile water.
Lower Part (Volumetric module)
The following components
only
can be immersed in a decontaminant solution: connecting nut, tip ejector,
tip holder.
Piston assembly and seals must be degreased with methyl alcohol before being immersed in
decontamination solution in separate vessel.
1.
Disassemble the pipette (refer to
MAINTENANCE FOR SINGLE AND FIXED MODELS
, page 14).
2. Immerse tip ejector, tip holder and connecting nut in the cleaning solution.
3. Degrease the piston assembly, the seals and immerse them in another vessel.
4. Rinse each component with distilled water.
5. Leave the parts to dry by evaporation (or wipe with a soft cloth the tip ejector, the tip holder and
connecting nut).
6. Lubricate the piston assembly and the seals.
7. Reassemble the piston assembly, the tip holder and the tip ejector.
FOR VARIABLE VOLUME MULTICHANNEL MODELS
The following components only can be immersed in a decontamination solution: tip ejector, ejector locks
and ejector spacer.
1.
Remove the tip ejector and the ejector spacer.
2. Immerse the tip ejector, ejector locks and ejector spacer in the decontamination solution or wipe
them with a soft-cloth or lint-free tissue soaked with the decontamination solution.
3. Rinse the components with distilled water.
4. Wipe the entire pipette with a soft cloth or lint-free tissue soaked
with the decontamination solution.
5. Wipe it with distilled water.
6. Leave the parts to dry by evaporation or wipe
them with a clean soft-cloth or lint-free tissue.
7. Refit the tip ejector (refer to
Change the Tip Ejector
, page 16).
Ejector spacer
Ejector lock
Tip ejector
