P
IP
E
T
TIN
G
10
PIPETTING
|
PIPETMAN L®
Aspirate
1.
Press the push button to the
first stop
(this
corresponds to the set volume of liquid).
2. Hold the pipette vertically and immerse the tip
in the liquid (see immersion depth table below).
Release the push button slowly and smoothly
(to top position) to aspirate the set volume of
liquid. Wait one second (time depends on model,
see table below) ; then withdraw the pipette tip
from the liquid. You may wipe any droplets away
from the outside of the tip using a medical wipe,
however if you do so take care to avoid touching
the tip’s orifice.
For the multichannel models, use a Gilson Reagent
Reservoir (Refer to
ACCESSORIES
on page 11).
First stop
Second stop
Figure 14
Pipetting Motion - Aspirate and Dispense
Dispense
1.
Place the end of the tip against the inside wall of the recipient vessel (at an angle of 10° to 40°).
2. Press the push button slowly and smoothly to the
first stop
.
3. Wait for at least a second, then press the push button to the
second stop
to expel any residual liquid
from the tip. Keep the push button pressed fully down and (while removing the pipette) draw the tip
along the inside surface of the vessel.
4. Release the push button, smoothly. Eject the tip by pressing firmly on the tip ejector button.
For the multichannel models, use a Gilson reagent reservoir (refer to
ACCESSORIES
on page 11).
Eject the Tips
Before you start to pipette, you can adjust the tip ejector button according to your preferences.
1.
Position the tip ejector button.
Simply rotate the tip ejector
button to the most comfortable
position: left, right, or middle.
2. Activate the tip ejector. You can
either push the tip ejector button
with the tip of the thumb as usual,
or with the base of your thumb
for more comfort. Please note
the P5000L and P10mL L are not
equipped with a tip ejector.
Guidelines for Good Pipetting
1.
Make sure that you operate the push button slowly and smoothly.
2. When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to the table).
MODEL
IMMERSION DEPTH
(MILLIMETERS)
WAIT TIME
(SECONDS)
P2L, P10L, P8x10L, P12x10L, F1L, F2L, F5L, F10L
1
1
P20L, P8x20L, P12x20L, P8x200L, P12x200L, F20L, F25L
2–3
1
P100L, P200L, P8x300L, P12x300L, F50L, F100L, F200L, F250L
2–4
1
P1000L, F300L, F400L, F500L, F1000L
2–4
2–3
P5000L
3–6
4–5
P10mL L
5–7
4–5
F5000L
3-6
1
Left
Middle
Right
Figure 15
Adjustable tip ejector button positions.
PIPETMAN L should be held in the vertical position.
NOTICE
