P
IP
E
T
TIN
G
8
PIPETTING
|
PIPETMAN L®
Graduations
Counterclockwise
Clockwise
To adjust the volume setting to compensate for a
specific density or viscosity, engage the two hooks
of the spanner tool inside the two small holes on the
top of the body, then turn the key slowly:
• Clockwise
to decrease the volume; making sure
not to overshoot the mark.
• Counterclockwise
to increase the volume; pass
the required value by 1/4 turn, then slowly decrease
the volume to reach the required setting.
Example: When using a PIPETMAN L Fixed 100 to aspirate
a particularly viscous solution, you may determine
gravimetrically that the volume delivered is 98.8 µL, and the
display reads “H”. Therefore, you wish to increase the volume
dispensed by 1.2 µL. As the interval between each letter on
the calibration display represents 0.2 µL for the F100L (1/10
of 2 µL), the calibration tool must be turned 3 units until the
display reads “B”.
It is possible to alter the volume by more than one full
turn in the counterclockwise direction. In this case,
remember to readjust the same number of turns when
it is necessary to return to the original setting.
Check the new volume gravimetrically. If the volumes
delivered are still not sufficiently close to the desired
value, make another slight adjustment in the relevant
direction.
Be sure to change tips between volume
setting adjustments and to pre-rinse each new tip.
When the PIPETMAN L Fixed is readjusted to
compensate for a particular solution, the nature of
the liquid can be noted on the labels inserted in the
slot under the counter.
Figure 7
Adjust PIPETMAN L Fixed volume setting to
compensate for a specific density or viscosity
Liquids of high density, vapor
pressure, or viscosity are best
pipetted by the MICROMAN
positive displacement pipette.
NOTICE
Figure 8
Identify the liquid used with the label
Chapter 4
PIPETTING
It is recommended to use PIPETMAN® DIAMOND Tips with the PIPETMAN L for optimum performance.
These tips are made from pure polypropylene. Plastic tips are for a single application—they must not be
cleaned for reuse.
Fit the Tips
SINGLE CHANNEL MODELS
To fit a new PIPETMAN DIAMOND tip, push the tip holder into the tip using a slight twisting motion to
ensure a firm and airtight seal.
For the P2L and P10L models equipped with a stainless steel tip ejector
, a dual-position adapter (plastic)
is required to fit DL10 tips (long tips) or D10 tips (short tips). The metallic rod of the tip ejector is shaped so
that the adapter may be clipped to it in either of two positions.
PIPETMAN DIAMOND Tip Compatibility
for Fixed models
F1L, F2L, F5L, F10L
D10, DL10, DF10, DFL10
F20L, F25L
D200, DF30
F50L, F100L
D200, DF100
F200L
D200, DF200
F250L
D300, DF300
F300L, F400L, F500L, F1000L
D1000, DF1000
F5000
D5000
PIPETMAN DIAMOND Tip Compatibility
for Single models
P2L, P10L
D10, DL10, DF10, DFL10
P20L
D200, DF30
P100L
D200, DF100
P200L
D200, DF200, D300, DF300
P1000L
D1000, D1200, DF1000, DF1200
P5000L D5000
P10mL L
D10mL
Figure 9
PIPETMAN DIAMOND Tip compatibility charts
