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PIPETMAN® M CONNECTED
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USER’S GUIDE
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BENEFIT FROM THE BLUETOOTH® CONNECTION
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PIPETMAN® M CONNECTED
Chapter 10
GUidELiNES FOr GOOd PiPETTiNG
Aspirate and Dispense
Fit new PIPETMAN DIAMOND Tip for the best results
(refer to Chapter 19
on page 31).
Single Channel Model
For single channel models, push the tip holder into the tip
using a slight twisting motion to ensure a firm and airtight
seal.
Multichannel Model
For multichannel models, PIPETMAN DIAMOND Tips are
best fitted with the ROCKY RACK technique, invented by
Gilson, available only in our TIPACK and TOWERPACK.
Pre-Wet the Tips
All Models
Pre-wetting the tips before pipetting helps prepare the tips for the best pipetting
performance. Ideally, the pre-wet includes both immersing the tip in the liquid and
performing one pipetting step. Pre-wetting the tips helps ensure that volumes that
you pipette will achieve accuracy and precision within specifications.
Hold the pipette vertically and immerse
the tip in the liquid (refer to Table 1).
Press the push button to aspirate the set
volume of liquid. Wait a few seconds (time
depends on model, refer to Table 1); then
withdraw the pipette tip from the liquid.
You may wipe any droplets away from
the outside of the tip using a medical
wipe; however if you do so, take care to
avoid touching the orifice of the tip.
Place the end of the tip against the inside
wall of the recipient vessel (at an angle of
10° to 40°). Press the push button. Wait
for at least a few seconds before releasing
the push button to expel any residual
liquid from the tip. While removing the
pipette draw the tip along the inside
surface of the vessel.
MODEL
IMMERSION
DEPTH (mm)
WAIT TIME
(SECONDS)
P10M
1
1
P20M
2-3
1
P100M
2-4
1
P200M
2-4
1
P300M
2-4
1
P1200M
2-4
2-3
P5000M
3-6
4-5
P10mLM
5-7
4-5
P8x10M/P12x10M
1
1
P8x20M/P12x20M
2-3
1
P8x100M/P12x100M
2-4
1
P8x200M/P12x200M
2-3
1
P8x300M/P12x300M
2-3
1
P8x1200M/P12x1200M
2-4
2-3
Table 1
Immersion depth and wait time
●
Mold and cavity references are marked on the collar, ensuring the traceability.
For quality assurance purposes batch numbers appear on all packages (bags and
boxes).
●
They form an airtight seal with the tip holder, preventing the leaks that cause poor
accuracy and a lack of precision.
●
PIPETMAN DIAMOND Tips (except filter tips) may be autoclaved at 121°C for
20 minutes at 0.1 MPa.
NOTICE
do not autoclave PiPETMAN Filter Tips as the filter will be damaged.
NOTE
To ensure the best performance from your Gilson pipette, you should
ALWAYS use PiPETMAN diAMONd Tips (in accordance with iSO8655)
system, because PiPETMAN diAMONd Tips were used to establish the
specifications.
Figure 7
Plastic adapter
for D10 and
DF10 tips
Figure 8
P8x10M and P12x10M
Long tips
Short tips
Ejector spacer
Ejector spacer D10
*A plastic adapter is required to eject
D10 and DF10 tips (short tips). The
adapter is supplied with P10M. No
adapter is required when using DL10
and DFL10 tips (long tips).
PIPETMAN M CONNECTED MULTICHANNEL
P8x10M
D10
DL10
DF10ST
DFL10ST
0.5–10 µL
0.5–10 µL
P12x10M
P8x20M
DL10
D200
DFL10ST
DF30ST
1–20 µL
1–20 µL
P12x20M
P8x100M
D200
DF100ST
10–100 µL
5–100 µL
P12x100M
P8x200M
D200
D300
DF100ST
DF200ST
DF300ST
20–200 µL
5–200 µL
P12x200M
P8x300M
D200
D300
DF200ST
DF300ST
10–300 µL
10–300 µL
P12x300M
P8x1200M
D1200
DF1200ST
50–1200 µL
50–1200 µL
P12x1200M
PIPETMAN M CONNECTED SINGLE CHANNEL
Model
PIPETMAN
DIAMOND Tips
Gilson Specification
Volume Range
Standard
PIPET Mode
REPETITIVE
Mode
P10M
D10*
DL10*
DF10ST
DFL10ST
0.5–10 µL
0.5–10 µL
P20M
D200
DF30ST
2–20 µL
2–20 µL
P100M
D200
DF100ST
5–100 µL
5–100 µL
P200M
D200
D300
DF200ST
DF300ST
20–200 µL
5–200 µL
P300M
D200
D300
DF200ST
DF300ST
20–300 µL
10–300 µL
P1200M
D1000
D1200
DF1000ST
DF1200ST
100–1200 µL
20–1200 µL
P5000M
D5000
500–5000 µL 100–5000 µL
P10mLM
D10mL
1–10 mL
200 µL–10 mL
Slot
