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PIPETMAN® M CONNECTED
|
USER’S GUIDE
PIPETTING MODES
|
PIPETMAN® M CONNECTED
2. Set the
MiX
volume: click the push button and set the volume by turning the push
button. Confirm your settings with a simple click. This is the volume that will be
aspirated and dispensed repeatedly.
“
MiX”
as long as you press the push button.
3. Fit a tip suitable for the model of PIPETMAN M Connected that you are using
(preferably use PIPETMAN DIAMOND Tips for assurance of accurate and precise
results; refer to Chapter 9
Mix
Press the push button; as long as you keep your thumb pressing the button, the pipette
continues mixing. Release the push button to complete the current mixing cycle.
Purge
Press the push button.
PUrGE
appears on the screen. Click again to purge and reset the
piston.
NOTICE
Always fit a tip before using any pipette!
Figure 4
MIX Mode
REVERSE Mode
The REVERSE Mode is reverse pipetting with a manual pipette. During
aspiration, additional liquid is added. After delivery, the excess volume
remains in the tip and is discarded.
The REVERSE Mode is ideal for pipetting viscous liquids, liquids with high vapor
pressure, or those that tend to foam. Your PIPETMAN M Connected aspirates a
selected volume and an excess. This excess compensates for the liquid that remains
as a film inside the tip during dispensing. For example: protein extraction, cell lysis,
plasmid isolation, cell culture, buffer preparation and, many others.
Electrophoresis Gels Loading Protocol
Electrophoresis gels loading protocol: first change the pipetting speed of your
pipette(refer to Chapter 8
on page 15). Aspiration can be
done by using the standard speed (by default speed 6). Dispensing should be done very
slowly and carefully, to prevent swirling and spilling of the samples, so select the lowest
speed 1.
1.
Press the menu button to access system menu, where you can choose between
different pipette modes. Select
“rEVErSE” Mode
by turning the push button.
To confirm your selection click, the push button.
Set the Volume
1.
Press the push button, set the volume by turning the push button and press again
to confirm your settings.
2. Fit a tip suitable for the model of PIPETMAN M Connected that you are using
(preferably use PIPETMAN DIAMOND Tips for assurance of accurate and precise
results; refer to Chapter 9
on page 15).
Aspirate
Press the push button to aspirate the selected
volume. An amount of liquid equal to the amount of
purged air is added. The volume aspirated will be a
little bit more than the set volume.
dispense
Press the push button to dispense the volume,
the additional amount of liquid remains in the tip.
Purge
Press the push button.
PUrGE
appears on the
screen. Press again to purge and reset the piston.
Keep your thumb pressing the push button until
the end of the purge. The piston reset takes place
after you release the push button again.
This feature enables you to remove the tip from
the liquid, without aspirating anything.
Eject tip
Press the ejection button.
NOTICE
Always fit a tip before using any pipette.
0
Purge
START button
Optional STOP
Start Purge
V (+ extra vol)*
Click to Purge
Figure 5
REVERSE Mode
NOTICE
if you purge without pulling out the tip, then air bubbles could get into
your gel!
