3. Click to confirm. Your pipette will be operational again.
Maintenance Operation for Single Channel Models
Tip holder and Tip Ejector
These parts must be changed if they are damaged. You may also
remove these parts for cleaning or decontamination purposes.
Changing the Tip Ejector
1.
Keep the tip ejector button depressed and grip the top of the tip
ejector with the other hand.
2. Gently rotate the tip ejector counterclockwise and separate its
connector from the operating rod.
3. Pull the tip ejector away from the body of the pipette.
4. Clean or autoclave the tip ejector and refit or replace it by reversing the procedure.
Changing the Tip holder (Lower Part)
After removing the tip ejector, you may remove the lower
part of the tip holder, which is more likely to become
contaminated or damaged than the upper part. Removal
of the lower part is shown in Figure 14; for the upper
part refer to
on page 26 (special
precautions are necessary).
1.
Gently rotate the lower part of the tip holder
counterclockwise to unscrew it from the upper part.
2. Separate the parts and remove the O-ring (refer to
Changing the Seal and/or O-ring
on page 26)
3. Clean, and if required, autoclave the lower part of the
tip holder (autoclaving: 20 min. at 121°C and 0.1MPa)
If required, lubricate the piston (refer to
on page 27) and fit a new
O-ring.
4. Screw the two parts together, making sure that the two parts are fully tightened, by hand.
5. Refit the tip ejector.
M
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24
PIPETMAN® M CONNECTED
|
USER’S GUIDE
CONFIGURATION
|
PIPETMAN® M CONNECTED
Maintenance Warning
PIPETMAN M Connected notifies you when
maintenance is due (Service alert is set by
default to
“On”). The following message
1
will appear:
After validation by a click, the following message
2
appears:
1.
If you click to confirm within 10 seconds. The pipette will automatically switch to
diSASSEMBLY
mode so you can carry out your maintenance safely.
2. Wait longer than 10 seconds and the option to have a reminder in one week will
appear
3
.
1
2
3
Piston
Tip holder
(lower)
Tip holder
(upper)
Figure 13
Ejector clip
Figure 14
Changing the tip holder
Adjustment
Press the menu button to enter the menu. Select
“CONFIGURATION”
by rotating the
push button and click to confirm your selection. Enter the sub-menu in the same way
and select the “
ADJUSTMENT”
menu.
The adjustment menu allows you to access the following items:
●
Standard readjustment
●
Reset settings
Standard Readjustment
This menu allows you to adjust the pipette using three calibration points: 10%, 50%,
and 100% of nominal volume (in according with ISO8655 recommendations).
You may want to calibrate your pipette for solutions with a density, viscosity, surface
tension or vapour pressure that are different than that of water. To return to the
factory settings, choose
reset settings
. Your PIPETMAN M Connected will be reset
to the three factory calibration values that are permanently stored in the pipette’s
firmware.
In accordance with ISO 8655 Gilson recommends a gravimetric procedure for
pipette calibration. This gravimetric method is used to establish the mean mass
of a given volume of water (taking into account evaporation losses, where
necessary). After converting the mean mass to a volume (using the Z factor, refer to
on page 36), enter the measured volumes(s) into the pipette’s
memory and the software readjusts the pipette accordingly. This method requires
the strict monitoring of environmental conditions and the use of routinely controlled
equipment that is adapted to the volume being measured.
Conversion to volume must take into account the density of the liquid as well as
evaporation during the cycle time. For each measurement, the corresponding volume
(
Vi
) can be calculated as follows:
Wi
is the weight as read on the balance
e
is the mean evaporation loss during the cycle
time
Z
expressed in µL/mg, is a conversion factor incorporating density of water or
other liquid buoyed in air, at test temperature and barometric pressure (refer to
on page 36)
For volumes greater than 50 μL, the evaporation factor can be disregarded.
Maintenance Operations
disassembly of your PiPETMAN M Connected to Change and Clean Parts
It is best to inspect your pipette regularly and to routinely clean and change parts
as required. To help you to keep up a regular schedule and in the interests of good
laboratory practices (GLP), you can configure your pipette to display an alarm before
servicing is due (refer to page 25).
Vi = (Wi + e) Z
NOTE
The following maintenance operation should only be done when the pipette
is in Service menu (refer to page 23).
NOTICE
After autoclaving the tip holder and tip ejector may change color, however
this has no impact on the performance.
