Dionex ICS-900, Operator'S Manual

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ICS-900 Operator’s Manual
46
Doc. 065215-02 9/09
3.11.5 Loading and Injecting Samples with a Syringe
This section describes two methods for loading sample into the injection
valve sample loop with a syringe:
•
Loading sample with a syringe through the sample loading port on the
front of the ICS-900 (push method)
•
Loading sample with a vacuum syringe through the sample loading
port on the front of the ICS-900 (pull method)
NOTE For instructions on how to load and inject
samples with an autosampler, see Section 3.11.4 .
Loading Samples with a Syringe (Push Method)
1. Verify that the sample loading port on the front of the ICS-900 is
connected to sample port
S (5)
on the injection valve (see
Figure 3-14 ).
2. Fill a syringe with a calibration standard or sample.
3. Insert the syringe into the sample loading port on the front of the
ICS-900.
4. Verify that the injection valve is in the Load position. If it is not, click
the Load button on the ICS-900 Control panel (see Figure 3-11 ).
5. Overfill the sample loop with five sample loop volumes. Remember
that the line from the syringe to the valve must be filled first before
the sample loop can be filled. Excess sample will exit through the
injection valve waste line.
6. Leave the syringe in the port. This prevents the sample from
siphoning out of the loop before injection.
7. Use one of the following methods to inject the sample onto the
column:
•
Manually: Click the Inject button on the ICS-900 Control panel.
See Section 3.11.2 for details about manually processing samples.
•
Automatically: Include the Inject command in a Chromeleon or
Chromeleon Xpress program. See the examples in
Section 3.11.6 .
See Section 3.11.3 for details about automatically processing
samples.