Covaris E220-plus Setup & Instruction Manual Download Page 1

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E220 & LE220 (-plus, R-plus, Rsc)

Setup & Instruction Manual

Instrument and Software Developed for High-performance

Sample Preparation using Adaptive Focused Acoustic

(AFA

) Energy

For Research Only

Model Numbers: E220-plus, E220R-plus, LE220-plus, LE220R-plus, and LE220Rsc | Software: SonoLab

10.2

Covaris

E220 & LE220 (-plus, R-plus, Rsc) Setup & Instruction Manual

Part Number: 010521 Rev B | May 2021

Summary of Contents for E220-plus

Page 1: ...or High performance Sample Preparation using Adaptive Focused Acoustic AFA Energy For Research Only Model Numbers E220 plus E220R plus LE220 plus LE220R plus and LE220Rsc Software SonoLab 10 2 Covaris...

Page 2: ...ers from Occupationally Acquired Infections Approved Guideline Fourth Edition published 2014 ISBN 1 56238 961 0 Print OSBN 1 56238 962 9 Electronic Occupational Safety and Health Administration OSHA 2...

Page 3: ...un a method without water in the bath since this could cause damage to the transducer The instrument is equipped with a water level sensor to protect the transducer and degassing pump The system will...

Page 4: ...and the medium on which the software is delivered is free of defects in materials under normal use and service The warranty is void if damage has resulted from third party software not intended for u...

Page 5: ...reate and Edit Methods 30 6 1 Create a New Method 30 6 2 Using the Repeat Function 36 6 3 Scanning Treatments LE220Rsc Only 39 6 4 Using Drag and Drop with Treatment Steps 42 6 5 Modify Existing Metho...

Page 6: ...n addition to the features of the E220 and LE220 plus described above the E220R and LE220R plus are designed to be integrated with a sample handling robot in an automated work cell The front door of t...

Page 7: ...AutoFill accessory kit Unpack the instrument the Water Conditioning System 2 0 WCS and the CH05 chiller Refer to the WCS User Manual to install the UV lamp into the WCS The lamp is shipped separately...

Page 8: ...d the remaining two ports on the opposite side of the WCS Connect the lower hose to the fitting on the left side of the water bath For the LE220Rsc the upper hose from the WCS 2 0 must connect to the...

Page 9: ...power source using the enclosed line cord The instrument is a universal input device and automatically employs any worldwide line voltage or frequency 100 to 240 volts AC 50 or 60 Hz Leave the instru...

Page 10: ...n Door button while pulling the door handle to open the safety enclosure front door Slide out the water bath and remove it from its plastic shipping bag Remove and discard the desiccant pack The instr...

Page 11: ...on the rear right side of the instrument at the power entry module When power is ON the green door lock release switch will illuminate and the ventilation fans in the rear panels will run Power on th...

Page 12: ...the SonoLab icon to start SonoLab The splash screen will appear Following the splash screen the motion system homing dialog appears Click Yes then OK when prompted to empty the sample tray The transd...

Page 13: ...n gray and inactive until a method is selected and all necessary conditions except the temperature and degassing states are met Your system automatically sets the temperature when a method is selected...

Page 14: ...rises beyond the user specified limit your system finishes the current position being treated pauses treatment and automatically resumes when the temperature is back within limits with no operator in...

Page 15: ...ry once a specific method is started Switches are provided in the Method Options pane to override the degas timer and temperature limits to allow your system to run a method without waiting for these...

Page 16: ...ged in the bath if automatic scheduling is active At the end of the scheduled period the scheduler will turn off the degas pump and disable cooling in the chiller then automatically restart both as sc...

Page 17: ...Duty Factor is set to 15 the Average Incident Power will be approximately 1 5 Watts However the real Average Incident Power from the transducer may vary due to factors such as sample tube type water...

Page 18: ...ter temperature to be within the range specified by the selected method Waiting for Water Degas the system waits for sufficient time for effective degassing to occur Waiting for Water to be Added to t...

Page 19: ...en initialized Door Closed a green check indicates the safety door is closed Water Degassed a green check indicates the water has been degassing for a sufficient length of time A yellow triangle indic...

Page 20: ...ors and user control functions Water System Indicators o Level Current indicates the current bath water level Minimum indicates the minimum level which will allow the currently selected method to run...

Page 21: ...tion through the WCS 2 0 to maintain water quality Method Options displays the Method Options panel which contains controls for overriding degassing or temperature parameters Override Temp when On the...

Page 22: ...ater bath level at the start of each treatment method However if the instrument will be idle for long periods of time during the day the Periodic Fill function can be configured to periodically check...

Page 23: ...be idle for long periods of time click the Enable Periodic Fill on off switch so that On is highlighted in dark blue SonoLab will automatically maintain the correct water level degassing and temperatu...

Page 24: ...ent files The Previous 100 button loads the next 100 more recent files The Go To control allows the operator to select files run on a specific date The files on the specified date will be displayed at...

Page 25: ...file which can be opened as a spreadsheet contains information about the methods run including times positions treated acoustic power bath water temperature and bath water level The Treatment file is...

Page 26: ...ault configuration requires no login to create run and edit methods User Authentication may be enabled to limit access to SonoLab functionality To enable User Authentication Start SonoLab Click the Lo...

Page 27: ...By default the Default user has Editor permissions access to all methods and Optional Consumable based Method Selection control See Section 5 5 2 below for details on users and their roles 5 5 2 Users...

Page 28: ...istory screens saved on the system 5 5 3 Managing Users To create a new user Log into SonoLab as Administrator and click on the ADMIN tab Click the New button at the upper right side of the screen The...

Page 29: ...lection can be selected by the user via the switch in the Method Options pane Refer to Section 5 1 for a description of the Method Pane and Appendix A for selecting and running methods with Consumable...

Page 30: ...ods will contain a single process with a single treatment step If more complex methods are required they can be easily constructed 6 1 Create a New Method 1 Click the New button on the METHOD screen T...

Page 31: ...Number 010521 Rev B May 2021 4 Click Process The process bar is displayed 5 Click the button inside the process bar to display the step type menu 6 Choose Treatment to specify the four acoustic param...

Page 32: ...type water level and water temperature The maximum Average Incident Power AIP the product of PIP times Duty Factor DF is limited to 250 watts on all E220 plus and LE220 plus instruments Cycles per Bur...

Page 33: ...e Duration range is from 0 to 60 seconds 7 Enter the desired dithering parameters if any and click OK 8 Enter the temperature set point for the protocol and the allowable range into the text entry fie...

Page 34: ...well plate Indexed treatment steps treat two columns at a time Scan steps treat one or more contiguous pairs of columns When using Position Restrictions columns are specified in a range of 1 to 12 no...

Page 35: ...fferent treatment steps The delay is specified in seconds 11 Add a Prompt Step if desired choose a Prompt step to display a message on the screen and pause execution of the method until the operator c...

Page 36: ...n Current Sample for E220R plus Indicates the Column Sample under treatment 6 2 Using the Repeat Function The Repeat Function is used when one or more treatments delays or prompt steps are to be appli...

Page 37: ...nario A Create a method with a Process step as explained previously Click the button inside the process bar to display the step type menu Click on Repeat Click the button inside the repeat bar to disp...

Page 38: ...ed to all selected columns samples of the plate once This repeats for the number of times specified in the Repeat bar Scenario B is typically used when a column sample is treated with an acoustic dose...

Page 39: ...3 Scanning Treatments LE220Rsc Only In a scanning treatment rather than the sample tray indexing to place one column of the sample tray at a time over the transducer the sample tray moves continuousl...

Page 40: ...ment dose in a scan treatment is a function of the scan speed and the number of scans With the Scan step outlined in orange click and drag on the columns to be treated Entire or partial plates may be...

Page 41: ...ic dose as the sample tray moves over the transducer in both directions Specifying 1 iteration in the Repeat step results in a single normal scan moving toward the front of the instrument If more than...

Page 42: ...rd steps Click and hold on the three bars at the left of the third step A Move Treatment box will hover over the mouse Drag the Move Treatment box over the first treatment step until a blue line shows...

Page 43: ...steps The screen is now configured as shown below When finished specify the samples to be treated and save the method as described in Section 6 1 above Behavior of Repeat steps is described in detail...

Page 44: ...0 Regulatory Labeling CE ETL Mark for Product Safety WEEE Safety Complies with Low Voltage Directive 2006 95 EC Certified to IEC EN ANSI UL 61010 1 2004 and CAN CSA C22 2 No 61010 1 2004 2nd Edition S...

Page 45: ...k the degas pump Off button in the Water Control pane 7 Click the Service button in the Motion Control pane The transducer rises out of the bath 8 Empty the bleach water solution 9 Place the empty wat...

Page 46: ...om the degassing pump lines Follow the System Shutdown procedure in Section 3 8 6 Cleaning the System Clean the external surfaces of the equipment as necessary with a damp lint free cloth Rinse the wa...

Page 47: ...Close SonoLab Exit SonoLab by clicking on the X in the upper right corner The following message is displayed Click Cancel to cancel this request Click Move and the transducer is moved to the service p...

Page 48: ...ustic Assembly is fully lowered into the bath Verify that the bath is slid fully into the enclosure so that the magnet embedded at the rear of the bath can be sensed Verify that the magnet is embedded...

Page 49: ...h a 10 bleach or equivalent solution if this has not recently been done Tube Breakage Verify that the acoustic parameters being employed are correct Verify that average power levels are approximately...

Page 50: ...able based Method Selection option is configured Always On or Always Off these setting take precedence and the switch in the Method Options pane is disabled See Managing Users in Section 5 5 3 for det...

Page 51: ...whether the instrument utilizes a line or point transducer Line transducers treat multiple samples in a column at a time The LE220 plus LE220R plus and LE220Rsc use a line transducer Point transducers...

Page 52: ...e is currently not supported All the numbers in position restrictions must be integers within the valid range of columns for the plate being used 1 to 12 for a 96 well plate for example All the letter...

Page 53: ...lower left of the screen The Data Converter pane is displayed Select which types of files Plate Definitions Methods or History by clicking their respective buttons to On All three file types are On b...

Page 54: ...uires about 5 0 liters of either deionized or distilled water which should be topped up daily The water should be changed and cleaning protocol with 10 bleach or equivalent solution performed at least...

Page 55: ...te Definition Files folder to C Users Public PublicDocuments Covaris SonoLab 10 Plate Definitions Install Windows Instrument Driver When an instrument with power ON is connected by USB Universal Seria...

Page 56: ...click the first device RF Controller Board and then select Update Driver Software When the driver software update dialog opens select the option to Browse my computer for driver software Point the fi...

Page 57: ...is now a USB Serial Port listed under the three RF Controller lines Right click on the USB Serial Port and select Update Driver Software as shown in the image below Repeat the installation steps shown...

Page 58: ...udes the need for a daily change of bath water It is however important that water which evaporates each day is made up with fresh distilled or deionized water It is also important that a monthly clean...

Page 59: ...water to the regulator the second piece connects the outlet of the regulator to the inlet of the particulate filter and the third piece connects the outlet of the particulate filter to the AutoFill in...

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