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Covaris
E220 & LE220 (-plus, R-plus, Rsc) Setup & Instruction Manual
Part Number: 010521 Rev B | May 2021
When the prompt step is reached during the treatment, the method pauses, and the prompt message will be displayed as
shown.
Example of a Prompt Message Display
12. Enter descriptive text into the “Creation Notes” section at the lower right side of the Method Editor window, if desired.
This field may be left blank.
13. When all method parameters and steps have been defined, click on the Save button to save changes. The user is prompted
to name the method.
14. Enter a name for the treatment method and click Save.
When the method is run, the ‘Current Column’ (‘Current Sample’ for E220R-plus) Indicates the Column (Sample) under treatment.
6.2 Using the Repeat Function
The Repeat Function is used when one or more treatments, delays, or prompt steps are to be applied to each column (sample in the
case of the E220R-plus) of the plate multiple times. There are two scenarios for how this may need to be performed, and the required
scenario determines how the repeat function is implemented.
In Scenario A, all treatment (or delay or prompt) steps are applied to the first column (sample) the number of times specified in the
Repeat bar, then all steps are applied to the next column (sample). This continues until the entire plate is treated.
Scenario A is used if each column (sample) requires one or more acoustic doses, but there is no need for resting the sample between
doses. Scenario A is also referred to as Pulsing.
